Evidence map›Paper›PMID 41552334›Full record

ArticleAmerican journal of translational research2025

Nanopore sequencing enables rapid diagnosis and drug resistance profiling in refractory

Yuwei Liu, Lin Xu, Yufei Chang, Xiaoyou Chen

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PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of translational research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yuwei LiuEmergency Department, Beijing Ditan Hospital, Capital Medical University Beijing 100015, China.
Lin XuPediatrics Department, Beijing Ditan Hospital Affiliated to Capital Medical University Beijing 100015, China.
Yufei ChangEmergency Department, Beijing Ditan Hospital, Capital Medical University Beijing 100015, China.
Xiaoyou ChenInfectious Diseases Department, Beijing Ditan Hospital, Capital Medical University Beijing 100015, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo evaluate the clinical value of nanopore sequencing technology (NST) for the early diagnosis and resistance guidance of

methodsWe analyzed four pediatric patients with MP pneumonia. Sputum specimens were subjected to NST, and results were compared with serological MP-IgM testing, nucleic acid detection, and chest CT.

resultsIn all four cases, MP-IgM yielded false-negative or weakly positive results within the first 5 days. In contrast, NST provided a definitive diagnosis within 24 hours of admission, detecting MP with sequence reads ranging from 14 to 3024 and simultaneously identifying the macrolide-resistant A2063G mutation in the 23S rRNA gene. This led to a confirmed diagnosis 3-7 days earlier than serological methods. Blood cultures were negative in all cases.

conclusionNST overcomes the limitations and delay of traditional methods, enabling rapid and accurate diagnosis of MP infection and concurrent detection of resistance mutations. Its integration into clinical practice may significantly improve the management of pediatric MP pneumonia.

Indexed as

childrendrug resistanceearly diagnosisMycoplasma pneumoniaenanopore sequencing

Identifiers

PMID41552334
PMCPMC12808016

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.