ArticleAmerican journal of translational research2025
Ferroptosis-related genes and pathways in knee osteoarthritis cartilage degeneration: discovered by bioinformatics technology and in vivo experimental verification.
Article in American journal of translational research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveThis study aimed to investigate the role of ferroptosis-related genes and pathways in cartilage degeneration in knee osteoarthritis (KOA) and to identify potential therapeutic targets.
methodsIntegrated bioinformatics analysis of public KOA transcriptomic datasets was performed to identify differentially expressed ferroptosis-related genes. Key pathways and hub genes were further validated using a surgically-induced KOA mouse model and clinical cartilage samples through histology, immunohistochemistry, micro-CT, ELISA, western blot, and qPCR.
resultsSignificant iron deposition, mitochondrial damage, and dysregulation of ferroptosis markers (e.g., decreased GPX4 and increased p53) were observed in KOA cartilage. Key genes such as MMP2, SPP1, MYC, and BNIP3 were identified, and pathways including mTOR and HIF-1 signaling were enriched. Experimental results consistently indicated enhanced ferroptosis and matrix degradation in both mouse and human OA samples.
conclusionFerroptosis contributes significantly to KOA progression through iron overload, lipid peroxidation, and dysregulated metabolic pathways, providing new insight for targeted therapeutic strategies.
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