ArticleCureus2025
Comparative Evaluation of IgM Enzyme-Linked Immunosorbent Assay (ELISA) and Real-Time PCR for the Early Diagnosis of Scrub Typhus in Patients With Febrile Illness.
Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Development of a Triplex TaqMan Real-Time PCR Assay for Simultaneous Detection of Duck Hepatitis B Virus, Duck Adenovirus Type 3, andVeterinary sciences · 2026Article
- Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionScrub typhus, caused by
methodsA cross-sectional study was conducted from January to April 2024. Blood samples were collected from 156 patients with febrile illness of less than 14 days. All samples were tested for scrub typhus IgM ELISA. ELISA-positive samples and a random selection of ELISA-negative, clinically suspected samples were subjected to RT-PCR for confirmation.
resultsOf the 156 patients, 46 (29.5%) were positive by IgM ELISA. Among these 46 ELISA-positive cases, 15 (32.6%) were positive by RT-PCR. A stark contrast was observed when analyzed by fever duration. Among the 15 RT-PCR positive cases, 100% had a fever duration of less than seven days. In contrast, only 17 (37.0%) of the 46 IgM ELISA-positive cases presented within the first seven days of fever (p<0.001). The 31 IgM ELISA-positive but RT-PCR-negative cases were predominantly from the later febrile phase (seven to 14 days).
conclusionsRT-PCR is a superior tool for the early diagnosis of scrub typhus within the first week of fever, while IgM ELISA becomes more reliable thereafter. A diagnostic approach utilizing RT-PCR in the initial febrile phase and serology in the later phase can significantly improve the accuracy and timeliness of scrub typhus diagnosis.
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