Evidence map›Paper›PMID 41550933›Full record

ArticleFrontiers in immunology2025

Single-cell RNA sequencing unraveled immune-related expression heterogeneity and lymphoid cell development dysregulation in childhood asthma.

Danying Zhu, Guang Li, Lang Yuan, Zeyu Zeng, Na Dong, Chao Wang, Ming Chen, Lijian Xie, Guohui Ding, Libing Shen and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Danying Zhu *Department of Respiratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Guang Li *Daozhi Precision Medicine Technology (Shanghai) Co., Ltd, Shanghai, China.
Lang Yuan *Department of Respiratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Zeyu ZengDepartment of Respiratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Na DongDepartment of Respiratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Chao WangDepartment of Respiratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Ming ChenDepartment of Respiratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Lijian XieDepartment of Pediatrics, Jinshan Hospital, Fudan University, Shanghai, China.
Guohui DingIntelligent Medicine Institute, Fudan University, Shanghai, China.
Libing ShenLonghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Xiaoyan DongDepartment of Respiratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Backgrounds: Asthma is a chronic inflammatory disease affecting airways, usually starting in childhood. Its cause remains unclear. Objective: We aim to elucidate the role of immune dysregulation in the pathogenesis of pediatric asthma. Methods: In this study, we used single-cell RNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from three pediatric asthma patients and four age-matched healthy controls to investigate the cellular etiology of childhood asthma. Results: The overall expression patterns of PBMCs from the three asthma patients indicate that both innate and adaptive immunity are imbalanced and abnormally activated in childhood asthma. Analysis of hematopoietic stem and progenitor cells (HSPCs) expression profiles further reveals that HSPCs from asthma patients tend to express immunity-related genes earlier. The cell developmental trajectories observed in asthma patients show an abnormal immune cell development pattern. Dysregulated lymphoid lineage development is observed in all three patients but there is no identical abnormal pattern for each patient. Pseudo-time analysis of gene expression demonstrates that JUN, a gene controlling cell cycle progression, is repressed in asthma patients while SPI1, an essential gene for lymphoid lineage development along with six inflammatory response related genes (S100A8, S100A9, S100A12, IL7R, IL32, and CCL5), exhibit various aberrant expression trajectories in asthmatic individuals. S100A8, S100A9, S100A12, and RETN are universally upregulated in various cell types of asthma patients. The analyses of cell-cell communication further elucidate the contributory roles of dendritic cells and CD14 Conclusions: Our scRNA-Seq analyses unveil childhood asthma as a complex disease with immune-related heterogenicities, characterized by dysregulated lymphoid cell development, a common feature that may offer a novel research direction for comprehensively understanding the key molecular mechanisms underlying childhood asthma.

Indexed as

AsthmaLymphocytesChildFemaleGene Expression ProfilingHematopoietic Stem CellsHumansImmunity, InnateLeukocytes, MononuclearMaleSequence Analysis, RNASingle-Cell AnalysisSingle-Cell Gene Expression Analysischildhood asthmahematopoietic stem and progenitor celllymphoid cellresistin (RETN)single-cell RNA sequencing

Identifiers

PMID41550933
PMCPMC12807962

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