Evidence map›Paper›PMID 41550878›Full record

ArticleThe journal of liquid biopsy2026

End-repair causes methylation underestimation in cell-free DNA sequencing libraries.

Tobin E Groth, Andrew A Mishin, Varsha Rao, Ruby Tibet, Christopher J Troll

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of liquid biopsy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tobin E GrothClaret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA, 95066, USA.
Andrew A MishinClaret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA, 95066, USA.
Varsha RaoClaret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA, 95066, USA.
Ruby TibetClaret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA, 95066, USA.
Christopher J TrollClaret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA, 95066, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free DNA methylation sequencing provides insight into tissue of origin and chromatin structure. In some workflows, generating libraries includes end-repair. Using matched single-stranded and double-stranded libraries prepared from the same cfDNA extracts, we show that end-repair in double-stranded DNA libraries reduces globally inferred CpG methylation leading to decreased tissue of origin accuracy. Trimming read termini partially mitigates this bias but decreases coverage and removes fragmentomic information compared to single-stranded DNA libraries, which forego end-repair.

Indexed as

Cell-free DNA (cfDNA)Double-stranded DNA (dsDNA) library preparationEnd-repairFragmentomicsMethylation sequencingNucleosome positioningSingle-stranded DNA (ssDNA) library preparationTissue of origin (TOO)

Identifiers

PMID41550878
PMCPMC12811460

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.