Evidence map›Paper›PMID 41550267›Full record

ArticleTransboundary and emerging diseases2026

Establishment and Evaluation of a Multicolor Latex Microsphere-Based Lateral Flow Immunoassay for the Simultaneous Detection of Antibodies Against African and Classical Swine Fever Viruses.

Jie Chen, Zhengwang Shi, Yi Ru, Juncong Luo, Qianqian Yang, Yage Xie, Lin Wang, Jing Zhou, Xiaoyang Zhang, Juanjuan Wei and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jie ChenState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.ORCID https://orcid.org/0009-0007-1548-5214
Zhengwang ShiState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.ORCID https://orcid.org/0000-0001-7941-8095
Yi RuState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Juncong LuoState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Qianqian YangState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Yage XieState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Lin WangState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Jing ZhouState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Xiaoyang ZhangState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Juanjuan WeiState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Yuqian ZhuState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.
Hong TianState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.ORCID https://orcid.org/0000-0002-1764-4916
Haixue ZhengState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.ORCID https://orcid.org/0000-0001-6850-1379
Nan-Hua ChenState Key Laboratory of Animal Disease Prevention and Control, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China, caas.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

African swine fever (ASF), a highly fatal disease often termed the "number one killer" of pigs, presents clinical symptoms indistinguishable from classical swine fever (CSF), such as fever, diarrhea, and vomiting, complicating on-site differential diagnosis. As both ASF and CSF are notifiable diseases under the World Organisation for Animal Health (WOAH), rapid and accurate identification is crucial for effective outbreak management. In this study, we developed a multicolor lateral flow immunoassay (LFIA) based on latex microspheres (LMs) for the simultaneous detection of antibodies against ASF virus (ASFV) and CSF virus (CSFV). The assay enables visual differentiation within 15 min, with red indicating ASFV antibodies and blue indicating CSFV antibodies. After optimization, the LFIA demonstrated a sensitivity of 1:256, equivalent to that of a commercial ASFV ELISA kit and four-fold higher than that for CSFV (1:64). The assay exhibited high specificity, showing no cross-reactivity with other common swine pathogens and bovine viral diarrhea virus (BVDV). When applied to 180 clinical serum samples and compared with commercial ELISA kits, the LFIA achieved Cohen's kappa values of 0.986 for ASFV and 0.918 for CSFV, indicating excellent agreement. Additionally, intra and interbatch evaluations confirmed its robust repeatability. Overall, the multicolor LM-LFIA offers a rapid, sensitive, specific, and cost-effective tool for point-of-care testing (POCT) of ASFV and CSFV antibodies, holding promise for routine field surveillance and disease control.

Indexed as

African Swine FeverAfrican Swine Fever VirusAntibodies, ViralClassical Swine FeverClassical Swine Fever VirusAnimalsImmunoassayLatexMicrospheresRapid Diagnostic TestsSensitivity and SpecificitySwineAntibodies, ViralLatexAfrican swine fever (ASF)classical swine fever (CSF)lateral flow immunoassay (LFIA)latex microsphere (LM)point-of-care testing (POCT)

Identifiers

PMID41550267
PMCPMC12809178

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.