Evidence map›Paper›PMID 41549998›Full record

ArticleJournal of cell science2026

Changes in nuclear and actin mechanics from G1 to G2 affect nuclear integrity.

Samantha Bunner, Katie Huang, Anish Shah, Schuyler Figueroa, Nick Lang, Catherine Chu, Nebiyat Eskndir, Mai Pho, Gianna Manning, Mindy Zheng and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Samantha BunnerBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Katie HuangBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Anish ShahBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Schuyler FigueroaBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nick LangBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Catherine ChuBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Nebiyat EskndirBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Mai PhoBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Gianna ManningBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Mindy ZhengBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Lilian Fritz-LaylinBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Katrina B VelleBiology Department, University of Massachusetts Dartmouth, North Dartmouth MA 02747, USA.
Joshua MarcusDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
James OrthMolecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO 80309, USA.
Andrew D StephensBiology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.ORCID 0000-0001-5474-7845

Funding

Role of chromatin mechanics in nuclear shape and integrityR35GM154928 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI Andrew Daniel Stephens · 2024 to 2026
$1.2M
Center for 3D Structure and Physics of the Genome 1UM1 HG011536Foundation for the National Institutes of Health R35GM154928NIGMS NIH HHS R35 GM154928NIH HHS R35GM154928University of Massachusetts Amherst
6 · The paper itself

Abstract

The structural integrity of the nucleus is dependent on nuclear mechanical elements of chromatin and lamins resisting antagonistic actin cytoskeleton forces. Force imbalance results in nuclear blebbing, rupture and cellular dysfunction found in many human diseases. Here, we used the fluorescent ubiquitin cell cycle indicator (FUCCI) cells to determine how cell cycle changes affect the nucleus and actin force balance. Whereas nuclear blebs were present equally throughout interphase, nuclear blebs formed predominantly in G1 and then persisted into G2. Actin-based nuclear confinement and focal adhesion density was greater in G1 versus G2 cells. Removal of focal adhesions through treatment with an inhibitor resulted in decreased nuclear confinement and blebbing, supporting this as the underlying mechanism. Upon artificial confinement, G2 nuclei ruptured more than G1 nuclei. Single nucleus micromanipulation force measurements confirmed that G1 nuclei were stiffer than G2 nuclei in both the chromatin-based and lamin-based nuclear stiffness regimes. Decreased nuclear stiffness can be explained by loss of peripheral H3K9me3 from G1 to G2, recapitulated by H3K9me3 inhibition through treatment with chaetocin. Cell cycle-based changes in nuclear and actin mechanics impact nuclear integrity and shape.

Indexed as

ActinsCell NucleusG1 PhaseG2 PhaseActin CytoskeletonHistonesHumansActinsHistonesActin confinementCell cycleG1G2HeterochromatinNuclear blebNuclear rupture

Identifiers

PMID41549998
PMCPMC12989073

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.