Evidence map›Paper›PMID 41547968›Full record

ArticleAnimal microbiome2026

Effects of dietary Bacillus amyloliquefaciens Y02 supplementation on the growth performance, immunity, intestinal morphology, and cecal microbiota of 1 to 42 days Langya chickens.

Lumin Yu, Shanpeng Zhang, Mengjie Yu, Yangzhe Zhao, Chao Wang, Yanyun Wang, Jiahao Wen, Chaojie Chen, Xinglin Zhang

Abstract read
In one paragraph

Article in Animal microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lumin YuCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China. yulumin@lyu.edu.cn.
Shanpeng ZhangCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
Mengjie YuCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
Yangzhe ZhaoCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
Chao WangCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
Yanyun WangCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
Jiahao WenCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
Chaojie ChenCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
Xinglin ZhangCollege of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China. zhangxinglin@lyu.edu.cn.

Funding

National Natural Science Foundation of China 32202810Natural Science Foundation of Shandong Province,China ZR2022QC115
6 · The paper itself

Abstract

backgroundBacillus amyloliquefaciens (B. amyloliquefaciens) has garnered significant attention and application as a feed additive in poultry production for improving the chickens’ production performance and intestinal health. However, it remains unclear whether B. amyloliquefaciens influences the production performance and intestinal health of Langya chickens, a Chinese local chicken breed. The present study aims to investigate the effect of dietary B. amyloliquefaciens supplementation on the growth performance, immune function, intestinal morphology, and cecal microbiota in Langya chickens.

resultsDietary supplementation with B. amyloliquefaciens Y02 had no effects on the final body weight, weight gain (WG), average daily gain (ADG), average daily feed intake (ADFI), and feed-to-gain ratio (F/G) of Langya chickens at 42 days of age in the treatment group (BAT) compared to the the control group (CON) (p > 0.05). However, the addition of B. amyloliquefaciens Y02 in the BAT group resulted in a 40% decrease in mortality of Langya chickens compared to the CON group. The serum immunoglobulin G (IgG) levels were increased by 31.56% and IgM by 32.36% in the BAT group in comparison to the CON group (p < 0.05), but tumor necrosis factor-α (TNF-α), interleukin-2 (IL), and IL-6 levels had no changes (p > 0.05). Compared to the CON group, the BAT group with B. amyloliquefaciens Y02 resulted in a 8.43% increase in the duodenal villus height (p < 0.01), a 24.13% increase in the duodenal villus height to crypt depth ratio (V/C) (p < 0.01), and a 15.83% decrease in the duodenal crypt depth (p < 0.05), as well as a 19.14% increase in the jejunal villus height (p < 0.01). Notably, the addition of B. amyloliquefaciens Y02 enriched the abundance of Bacteroides, Clostridum, and Alistipes at the genus level, resulting in the production of certain beneficial metabolites that play pivotal roles in maintaining the intestinal health, promoting the immune function, and reducing the colonization of harmful bacteria.

conclusionsThis study demonstrates that B. amyloliquefaciens Y02 has many beneficial effects in terms of enhancing the immune defense mechanisms, improving the intestinal morphology, altering the cecal microbial composition, and maintaining the cecal microbiota balance in Langya chickens. CLINICAL TRIAL NUMBER: Not applicable.

Indexed as

Bacillus amyloliquefaciensCecal microbiotaImmune functionIntestinal morphologyLangya chickens

Identifiers

PMID41547968
PMCPMC12895774

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.