Evidence map›Paper›PMID 41546044›Full record

ArticlePlant methods2026

Cross-species optimization of nuclei isolation in ten plant species.

Yun Luo, Jiali Yan, Thuy La, Edward S Buckler, Jianbing Yan, M Cinta Romay

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yun Luo *Institute for Genomic Diversity, Cornell University, Ithaca, NY, 14853, USA. yl3956@cornell.edu.
Jiali Yan *National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
Thuy LaInstitute for Genomic Diversity, Cornell University, Ithaca, NY, 14853, USA.
Edward S BucklerInstitute for Genomic Diversity, Cornell University, Ithaca, NY, 14853, USA.
Jianbing YanNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
M Cinta RomayInstitute for Genomic Diversity, Cornell University, Ithaca, NY, 14853, USA.

Funding

National Natural Science Foundation of China 32301840
6 · The paper itself

Abstract

Single-cell technologies are transforming plant biology, yet broadly transferable nuclei isolation remains a key bottleneck for snRNA-seq. We developed a reproducible, cost-efficient Percoll-based workflow that is applicable to multiple maize tissues and nine additional plant species. In maize, nuclei from root, shoot, leaf, and embryo consistently concentrated at the 80% Percoll interface and exhibited high integrity, with typical recoveries > 50,000 nuclei per sample. For other species, gradient compositions were tuned according to genome size to achieve efficient enrichment and clean suspensions, and yields ranged from 17,000 to 40,000 nuclei per sample. Downstream validation showed that nuclei from special interest maize and Tripsacum generated high-quality snRNA-seq libraries, as supported by cDNA quality profiles. These results demonstrate the versatility and robustness of the method across species and tissues.

Indexed as

MaizeNuclei isolationPercoll gradientPlant tissuesSingle-nucleus RNA sequencing (snRNA-seq)Tripsacum

Identifiers

PMID41546044
PMCPMC12892638

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.