Evidence map›Paper›PMID 41545441›Full record

ArticleScientific reports2026

Evaluation of polyclonal antibodies raised in rabbits against dengue NS1 antigen.

Philip Raj Abraham, Panneer Devaraju, Bharath L, Eiriene Prasad, Vidhya Shree S, Akash Dhotre, Naveen Kumar Ramachandrappa, Surekha Mullapudi Venkata, Ashwani Kumar

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

9 authors.

Philip Raj AbrahamICMR-Vector Control Research Centre, Puducherry, India. microphilip_14@rediffmail.com.ORCID https://orcid.org/0000-0003-4584-8139
Panneer DevarajuICMR-Vector Control Research Centre, Puducherry, India. panneeryadav82@gmail.com.ORCID http://orcid.org/0000-0003-0638-9590
Bharath LICMR-Vector Control Research Centre, Puducherry, India.
Eiriene PrasadICMR-Vector Control Research Centre, Puducherry, India.
Vidhya Shree SICMR-National Institute of Nutrition, Hyderabad, India.
Akash DhotreICMR-Vector Control Research Centre, Puducherry, India.
Naveen Kumar RamachandrappaICMR-National Institute of Nutrition, Hyderabad, India.
Surekha Mullapudi VenkataICMR-National Institute of Nutrition, Hyderabad, India.
Ashwani KumarICMR-Vector Control Research Centre, Puducherry, India.

Funding

Indian Council of Medical Research IM-1903
6 · The paper itself

Abstract

Dengue, a vector-borne disease caused by the dengue virus (DENV), is spread by Aedes mosquitoes like, Aedes aegypti and Aedes albopictus. In the absence of antiviral drugs or vaccines, dengue prevention is centered on mosquito vector control. Laboratory confirmation of DENV infection is essential to offer the appropriate clinical care. The non-structural protein-1 (NS1) antigen of the virus, in its secretory form, is being employed as a diagnostic marker to identify active dengue infection in humans and mosquito vectors. Commercial dengue NS1 ELISA kits use monoclonal antibodies to detect the NS1 antigen. However, polyclonal antibodies offer several advantages over monoclonal antibodies in terms of shorter production time, lower cost and high stability. Therefore, in the present study, we have raised Rabbit Polyclonal Antibodies against the Dengue NS1 antigen (RPAD) and evaluated their sensitivity and specificity in detecting dengue virus. The RPAD were tested against commercial recombinant dengue virus-2 NS1 antigen (rDNS1Ag) by standardized ELISA and Western Blot assays. Further, the sensitivity of RPAD was checked against the NS1 antigens of different serotypes of DENV. The specificity of RPAD towards the NS1 antigens of other flaviviruses was also tested. The RPAD demonstrated consistent immunoreactivity toward recombinant NS1 antigens of all four dengue virus serotypes in ELISA and Western blot assays, with minimal cross-reactivity to NS1 antigens of other flaviviruses. These findings indicate the potential utility of RPAD as a laboratory reagent for NS1 antigen detection, warranting further validation using clinical samples and field-collected mosquito vectors.

Indexed as

AedesAntibodies, ViralDengueDengue VirusAnimalsEnzyme-Linked Immunospot AssayHumansMaleMiceMice, Inbred BALB CRabbitsViral Nonstructural ProteinsAntibodies, ViralViral Nonstructural ProteinsAedes aegyptiDengue virusNS1 antigenPolyclonal antibodyVector-borne disease

Identifiers

PMID41545441
PMCPMC12891463

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