An artificial cell capable of signal transduction mediated by ADRB2 for the regulation of glycogenolysis.

Yanhao Liu, Wan Zhao, Yingming Zhao, Xiangxiang Zhang, Jingjing Zhao, Shubin Li, Yongshuo Ren, Xiaojun Han

Abstract read

In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yanhao LiuState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.

Wan ZhaoState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.

Yingming ZhaoState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.

Xiangxiang ZhangState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.

Jingjing ZhaoState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.

Shubin LiState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China. lishubin@hit.edu.cn.

Yongshuo RenState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.

Xiaojun HanState Key Laboratory of Urban-rural Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China. hanxiaojun@hit.edu.cn.ORCID http://orcid.org/0000-0001-8571-6187

Funding

National Natural Science Foundation of China (National Science Foundation of China) No. 22174031National Natural Science Foundation of China (National Science Foundation of China) No. 22374033Natural Science Foundation of Heilongjiang Province No. ZD2022B001

6 · The paper itself

Abstract

Bottom-up construction of artificial cells helps elucidate the working mechanism of cells. Signal transduction from extracellular to intracellular artificial cells is essential for autonomous artificial cells. It remains highly challenging to reconstitute G protein-coupled receptor (GPCR) signaling pathways to regulate downstream metabolism in artificial cells. Here, we reconstitute β2-adrenergic receptor, Gs subunit α and adenylate cyclase V into artificial cell membranes to enable signal transduction from extracellular isoproterenol (ISO) to intracellular cAMP (visualization via Epac1-cAMP probes). cAMP production is ISO dose-dependent, with a maximum amplification fold of 22.45 ± 2.14. By encapsulating the glycogenolytic pathway, cAMP activates protein kinase A, triggering phosphorylation of phosphorylase kinase and glycogen phosphorylase to convert glycogen to glucose-1-phosphate (G-1-P). G-1-P is further converted to 6-phosphogluconolactone accompanying with NADPH. ISO stimulation induces G-1-P and NADPH generation, achieving progressive signal amplification. The successful reconstitution of GPCR-mediated signaling pathway in artificial cells paves the way for developing autonomous artificial cells.

Indexed as

GlycogenolysisReceptors, Adrenergic, beta-2Signal TransductionAdenylyl CyclasesAnimalsCyclic AMPCyclic AMP-Dependent Protein KinasesGlycogenGTP-Binding Protein alpha Subunits, GsHumansIsoproterenolPhosphorylationAdenylyl CyclasesCyclic AMPCyclic AMP-Dependent Protein KinasesGlycogenGTP-Binding Protein alpha Subunits, GsIsoproterenolReceptors, Adrenergic, beta-2

Identifiers

PMID41545427

DOI10.1038/s41467-026-68503-3

PMCPMC12916806

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.