Evidence map›Paper›PMID 41545422›Full record

ArticleScientific reports2026

Molecular insights on the proangiogenic effects of VEGF like growth factor derived from horseshoe crab perivitelline fluid.

K G Aghila Rani, Hrishikesh Pandit, Rambhadur Subedi, Sayli Otiv-Pandit, Anil Bankati Jogdand, Hajra Gupta, Prayagraj Fandilolu, Sushama Rokade, Susan Idicula-Thomas, Anil Chatterji and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

K G Aghila Rani *Department of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India.
Hrishikesh Pandit *Department of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India.
Rambhadur Subedi *Department of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India.
Sayli Otiv-PanditDepartment of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India.
Anil Bankati JogdandDepartment of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India.
Hajra GuptaDepartment of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India.
Prayagraj FandiloluBiomedical Informatics Center, ICMR-National Institute for Research in Reproductive and Child Health, J. M. Street, Parel, Mumbai, 400 012, India.
Sushama RokadeDepartment of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India.
Susan Idicula-ThomasBiomedical Informatics Center, ICMR-National Institute for Research in Reproductive and Child Health, J. M. Street, Parel, Mumbai, 400 012, India.
Anil ChatterjiMalkolak Institute of Marine Sciences, 8 Astha Society, Bella Vista Colony, Dona Paula, Goa, 403 004, India.
Taruna MadanDepartment of Innate Immunity, National Institute for Research in Reproductive and Child Health (ICMR), J. M. Street, Parel, Mumbai, 400 012, India. guptat@nirrch.res.in.

Funding

Department of Biotechnology, Government of India BT/PR40165/BTIS/137/12/2021
6 · The paper itself

Abstract

The current study investigated the proangiogenic effects of fraction VII of perivitelline fluid (hscPVF-VEGF) obtained from the late-stage embryos of Indian horseshoe crab (Tachypleus gigas; Müller) using human umbilical cord tissue-derived MSCs (hUCMSCs). Angiogenic potential of hscPVF-VEGF was investigated by analyzing transcripts of signature angiogenic markers, key transcription factors and matrix metalloproteases. Molecular docking studies were performed to predict the binding site of hscPVF-VEGF with the VEGF receptor (VEGFR). hscPVF-VEGF significantly upregulated VEGF, vWF, and downregulated sFlt-1. Significant increase in transcriptional levels of HOXA7, HOXB3, HOXB5, CD31, MMP2, and MMP9 further elucidated the molecular mechanism underlying the angiogenic ability of hscPVF-VEGF. Wound healing assay revealed the migratory potential of hscPVF-VEGF. Molecular docking studies predicted that hscPVF-VEGF may modulate hVEGFR activity by binding in a pocket within the extracellular domains (D5, D6, and D7) distal to the VEGF binding site (D2 and D3). This study infers the potential and molecular mechanism of hscPVF-VEGF inducing angiogenic differentiation in hUCMSCs, suggesting clinical application of a recombinant form of hsPVF-VEGF in disorders with dysfunctional angiogenesis.

Indexed as

AngiogenesisHorseshoe CrabsNeovascularization, PhysiologicVascular Endothelial Growth Factor AAnimalsBinding SitesCell MovementHumansMesenchymal Stem CellsMolecular Docking SimulationUmbilical CordVascular Endothelial Growth Factor AAngiogenesisCell migrationHUCMSCsPVFVEGFR

Identifiers

PMID41545422
PMCPMC12824273

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.