Evidence map›Paper›PMID 41543605›Full record

ArticleCell and tissue research2026

Characterization of bovine oviductal organoids: Polarity, cryopreservation, hormonal stimulation, and extracellular vesicles.

Riley E Thompson-Brandhagen, Mindy A Meyers, Brandi Dunn, Nico G Menjivar, Jennifer Palmer, D N Rao Veeramachaneni, Dawit Tesfaye, Fiona K Hollinshead

Abstract read
PubMed Publisher
In one paragraph

Article in Cell and tissue research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Multiscale imaging of polarity in bovine oviductal organoids.Frontiers in cell and developmental biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Riley E Thompson-BrandhagenDepartment of Clinical Sciences, Colorado State University, Fort Collins, CO, USA. riley.thompson2@colostate.edu.
Mindy A MeyersDepartment of Clinical Sciences, Colorado State University, Fort Collins, CO, USA.
Brandi DunnAnimal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO, USA.
Nico G MenjivarAnimal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO, USA.
Jennifer PalmerAnimal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO, USA.
D N Rao VeeramachaneniAnimal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO, USA.
Dawit TesfayeAnimal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO, USA.
Fiona K HollinsheadDepartment of Clinical Sciences, Colorado State University, Fort Collins, CO, USA.

Funding

120 kV Transmission Electron Microscope for CU Anschutz Electron Microscopy Core FacilityS10OD036258 · OD · UNIVERSITY OF COLORADO DENVER · PI FARHANG DAREHSHOURI, ANZA · 2024 to 2024
$678k
NIH HHS S10 OD036258
6 · The paper itself

Abstract

The oviduct is an important reproductive organ that fosters fertilization and early embryo development in mammals. With growing utilization of assisted reproductive technologies (ARTs) in animal agriculture, recent advancements in cell culture modeling have the potential to optimize in vitro fertilization methods. Organoids are a three-dimensional (3D) cell culture model poised to serve a key role in our understanding of endogenous oviductal physiology in cattle. However, limited information is currently available for the use of bovine oviductal organoids. Thus, the objective of our study was to conduct a comprehensive characterization of bovine oviductal organoids including polarity, cryopreservation, responsiveness to hormonal cues, and extracellular vesicle assessment. We observed that bovine oviductal organoids have the capacity for culture longevity for at least 86 days with or without cryopreservation. Furthermore, the organoid polarity can be manipulated by presence or absence of an extracellular matrix, and they respond to stimulation with hormones associated with estrus and diestrus through gene expression changes and extracellular vesicle secretion. In conclusion, bovine organoids display similar characteristics to in vivo oviductal cells and constitute a cell culture model in which to study oviductal physiology to improve ARTs in beef and dairy agriculture.

Indexed as

Cell PolarityCryopreservationExtracellular VesiclesHormonesOrganoidsOviductsAnimalsCattleFemaleHormones3D cultureExosomeFallopian tubeMicrovesicleReproduction

Identifiers

PMID41543605

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.