Evidence map›Paper›PMID 41543236›Full record

ArticleBiotechnology progress

Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions.

Akshay S Chaubal, Asingsa W Arachchige, Annabelle J Zahn, S Ranil Wickramasinghe, Xianghong Qian, Andrew L Zydney

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Akshay S ChaubalDepartment of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0009-0000-5647-2443
Asingsa W ArachchigeDepartment of Chemical Engineering, University of Arkansas, Fayetteville, Arkansas, USA.ORCID 0009-0001-7321-6932
Annabelle J ZahnDepartment of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania, USA.
S Ranil WickramasingheDepartment of Chemical Engineering, University of Arkansas, Fayetteville, Arkansas, USA.ORCID 0000-0001-7436-0517
Xianghong QianDepartment of Biomedical Engineering, University of Arkansas, Fayetteville, Arkansas, USA.
Andrew L ZydneyDepartment of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0000-0003-1865-9156

Funding

National Science Foundation 2310832National Science Foundation 2310905National Science Foundation RII 2218054
6 · The paper itself

Abstract

As adeno-associated viral vectors (AAV) continue to advance through the clinical pipeline, effective downstream purification strategies must be developed to ensure bulk drug purity and safety. AAV are produced within mammalian cells, bringing forth risks associated with viral contamination. Although existing downstream operations provide some degree of viral inactivation and removal, regulatory agencies have recommended the incorporation of a dedicated virus removal filtration step to ensure robust viral clearance. Recently published studies have demonstrated that membrane filters with nominal pore sizes between 35 and 50 nm can provide effective AAV transmission while removing larger viruses, although these results were obtained over a limited range of conditions. This study represents the first investigation into the effects of filtrate flux and process disruptions on virus reduction filtration for AAV. Experiments were performed using purified AAV capsids and carboxylate-modified polymeric nanoparticles with a nominal diameter of 20 nm. Initial results confirmed that both systems exhibited nearly identical transient transmission profiles during virus filtration. Virus filtration performed at various filtrate fluxes (between 20 and 185 L/m

Indexed as

DependovirusFiltrationGenetic VectorsAnimalsHumansadeno‐associated virus (AAV)downstream processinginternal polarizationprocess disruptionsviral clearancevirus filtration

Identifiers

PMID41543236
PMCPMC13055148

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.