Evidence map›Paper›PMID 41543137›Full record

ArticleThe plant genome2026

Genomic architecture of the resistance to Phytophthora cactorum 2 (RPc2) locus in strawberry (Fragaria × ananassa).

Hyeondae Han, Youngjae Oh, Yoon Jeong Jang, Saket Chandra, Upinder Gill, Sujeet Verma, Young-Hee Noh, Sachiko Isobe, Dominique D A Pincot, Glenn S Cole and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The plant genome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Plants (Basel, Switzerland) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Hyeondae HanGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.ORCID https://orcid.org/0000-0002-4657-5016
Youngjae OhGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.
Yoon Jeong JangGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.ORCID https://orcid.org/0000-0002-7119-7117
Saket ChandraGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.
Upinder GillGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.ORCID https://orcid.org/0000-0003-4572-5243
Sujeet VermaGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.
Young-Hee NohGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.
Sachiko IsobeKazusa DNA Research Institute, Kisarazu, Japan.
Dominique D A PincotDepartment of Plant Sciences, University of California, Davis, Davis, California, USA.ORCID https://orcid.org/0000-0001-6463-1213
Glenn S ColeDepartment of Plant Sciences, University of California, Davis, Davis, California, USA.
Randi A FamulaDepartment of Plant Sciences, University of California, Davis, Davis, California, USA.ORCID https://orcid.org/0000-0001-8490-1989
Mitchell J FeldmannDepartment of Plant Sciences, University of California, Davis, Davis, California, USA.ORCID https://orcid.org/0000-0002-7257-3401
Steven J KnappDepartment of Plant Sciences, University of California, Davis, Davis, California, USA.ORCID https://orcid.org/0000-0001-6498-5409
Vance M WhitakerGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.
Seonghee LeeGulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, Florida, USA.

Funding

Cooperative Research Program of Agriculture Science and Technology Development PJ017707National Institute of Food and Agriculture 2017-51181-26833National Institute of Food and Agriculture 2022-51181-38328Plant Health and Production and Plant Products: Plant Breeding for Agricultural Production 2024-67013-42583RDA Fellowship Program of the National Institute of Horticultural and Herbal Science, Rural Development Administration, Republic of Korea
6 · The paper itself

Abstract

Phytophthora crown rot, caused by Phytophthora cactorum, is a soilborne disease with broad impacts on cultivated strawberry (Fragaria × ananassa). A resistance locus, RPc2, was previously identified in octoploid strawberries, but the underlying genomic architecture and causal genes remained uncharacterized. In this study, we fine-mapped RPc2 from a 1.12 Mb interval to a 546 kb region containing 92 genes by genotyping an association panel of 339 accessions with high-resolution subgenome-specific markers. To discover candidate genes, a chromosome-scale haplotype-phased genome of the resistant breeding line FL16.33-8 was assembled and validated with a high-density genetic map and comparative analyses with high-quality phased octoploid reference genomes, Florida Brilliance and Royal Royce. Transcriptome profiling following pathogen inoculation combined with comparative genomics identified wall-associated kinase 1 (WAK1) and cyclic nucleotide-gated channel (CNGC1 CNGC2) as strong candidates within the RPc2 region. Using an efficient Agrobacterium-mediated transient expression assay, we confirmed their role in resistance to P. cactorum in strawberry. RNA interference knockdown of these genes increased disease severity, while overexpression of WAK1 or CNGC1 reduced crown rot symptoms in susceptible backgrounds. Furthermore, population analyses of 1029 global accessions revealed that the predominant-resistant haplotype (RPc2-H3) is common in modern cultivars and shows evidence of positive selection in breeding programs. Taken together, our study refines the genomic region of RPc2, identifies and functionally validates WAK1 and CNGC1/2 for the resistance to Phytophthora crown rot, and demonstrates that RPc2-H3 has been selected globally. These findings from this study would facilitate the advancement of genome-assisted breeding strategies for resistance to Phytophthora crown rot in strawberry.

Indexed as

Disease ResistanceFragariaGenome, PlantPhytophthoraPlant DiseasesChromosome Mapping

Identifiers

PMID41543137
PMCPMC12823032

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.