Evidence map›Paper›PMID 41543019›Full record

ArticleReproduction & fertility2026

Optimizing fixed-time laparoscopic artificial insemination in the jaguar.

Lindsey Marie Vansandt, Cristina Harumi Adania, Priscila Rocha Yanai, Jéssica da Silva Paulino, Regina Celia Rodrigues da Paz, Helen L Bateman, Elizabeth Marie Donelan, William F Swanson

Abstract read
In one paragraph

Article in Reproduction & fertility, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lindsey Marie VansandtCenter for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, Cincinnati, Ohio, USA.ORCID 0000-0002-5951-2057
Cristina Harumi AdaniaCentro Brasileiro Para Conservação dos Felinos Neotropicais, Associação Mata Ciliar, Jundiaí, SP, Brazil.
Priscila Rocha YanaiCentro Brasileiro Para Conservação dos Felinos Neotropicais, Associação Mata Ciliar, Jundiaí, SP, Brazil.ORCID 0000-0002-5744-7490
Jéssica da Silva PaulinoCentro Brasileiro Para Conservação dos Felinos Neotropicais, Associação Mata Ciliar, Jundiaí, SP, Brazil.
Regina Celia Rodrigues da PazLaboratório de Pesquisa em Animais de Zoológico, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil.ORCID 0000-0003-4567-0043
Helen L BatemanCenter for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, Cincinnati, Ohio, USA.ORCID 0000-0001-5135-8887
Elizabeth Marie DonelanCenter for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, Cincinnati, Ohio, USA.ORCID 0000-0002-4122-2406
William F SwansonCenter for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, Cincinnati, Ohio, USA.ORCID 0000-0003-4067-0319

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Abstract: The jaguar faces significant threats to survival from habitat fragmentation/loss and poaching. Conservation efforts include maintaining ex situ populations in zoos, but breeding success remains limited, necessitating the development of assisted reproductive technologies. This study aimed to optimize ovarian synchronization and artificial insemination (AI) protocols for jaguars by evaluating ovarian follicular activity during oral altrenogest treatment and in response to equine chorionic gonadotropin (eCG)/porcine luteinizing hormone (pLH) regimens, and assessing fertility outcomes with laparoscopic AI. In experiment 1, females were fed altrenogest (0.044 or 0.088 mg/kg, n = 2 per dose) daily for 45 days, but neither dose suppressed ovarian activity. In experiment 2, females received 0.088 or 0.176 mg/kg altrenogest (n = 2 per dose) for 45-48 days and then the alternate dose six months later. After a 5-day withdrawal, 600 IU eCG and 5,000 IU pLH (82 h interval) were administered intramuscularly. All eight cycles produced multiple follicles, but ovulation rates were low and no pregnancies occurred. In experiment 3, five females received 0.176 mg/kg altrenogest, followed by a 7-day withdrawal, 600 IU eCG, and 10,000 IU pLH (90-92 h interval). All females achieved multiple ovulations, and one female conceived, delivering a live cub after 103 days - the first jaguar ever produced from AI. In summary, altrenogest and eCG/pLH treatment following species-specific adjustments was effective for ovarian synchronization in the jaguar. The birth of a live cub following AI validates the effectiveness of this hormone regimen and highlights the potential of AI for population management of this imperiled felid.

Indexed as

Insemination, ArtificialLaparoscopyPantheraAnimalsChorionic GonadotropinEstrus SynchronizationFemaleLuteinizing HormoneOvarian FolliclePregnancyTrenbolone AcetatealtrenogestChorionic GonadotropinLuteinizing HormoneTrenbolone Acetatealtrenogestartificial inseminationassisted reproductive technologiesequine chorionic gonadotropinporcine luteinizing hormone

Identifiers

PMID41543019
PMCPMC13138596

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.