Evidence map›Paper›PMID 41542533›Full record

ArticlebioRxiv : the preprint server for biology2026

Stepwise DNA unwinding gates TnpB genome-editing activity.

Zehan Zhou, Iren Saffarian-Deemyad, Honglue Shi, Trevor Weiss, Muhammad Moez Ur-Rehman, Kamakshi Vohra, Petr Skopintsev, Peter H Yoon, Marena I Trinidad, Conner J Langeberg and 14 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

24 authors.

Zehan ZhouInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Iren Saffarian-DeemyadDepartment of Physics, Stanford University, Stanford, CA, USA, 94305.
Honglue ShiInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.ORCID 0000-0003-3847-1652
Trevor WeissDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Muhammad Moez Ur-RehmanInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Kamakshi VohraInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Petr SkopintsevInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Peter H YoonInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Marena I TrinidadInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Conner J LangebergInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.ORCID 0000-0002-5609-3758
Maris KamaluDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Jasmine AmerasekeraDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Erin E DohertyInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.ORCID 0000-0002-1555-4124
Kevin D P ArisDepartment of Bioengineering, Stanford University, Stanford, CA, USA, 94305.
Noor Al-SayyadDepartment of Physics, Stanford University, Stanford, CA, USA, 94305.
Brittney W ThorntonInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Rachel F WeissmanInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.ORCID 0000-0002-1806-6564
Kevin M WaskoInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Isabel Esain-GarciaInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Evan C DeTurkInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
David F SavageInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.
Steven E JacobsenDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA, 90095.
Zev BryantDepartment of Bioengineering, Stanford University, Stanford, CA, USA, 94305.
Jennifer A DoudnaInnovative Genomics Institute, University of California, Berkeley, CA, USA, 94720.ORCID 0000-0001-9161-999X

Funding

Structural Dynamics and Mechanochemical Coupling in Nucleoprotein MachinesR01GM106159 · NIGMS · STANFORD UNIVERSITY · PI Zev Bryant · 2014 to 2026
$3.2M
The dynamics, functions and evolution of a miniature RNA-guided enzymeK99GM160778 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Honglue Shi · 2025 to 2026
$240k
Precision Deaminases to Expand the Scope of Diseases Amenable to CRISPR-mediated CorrectionF32GM153031 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Erin E Doherty · 2024 to 2026
$229k
NIGMS NIH HHS F32 GM153031NIGMS NIH HHS K99 GM160778NIGMS NIH HHS R01 GM106159
6 · The paper itself

Abstract

TnpB is a compact RNA-guided endonuclease and evolutionary ancestor of CRISPR-Cas12 that offers a promising platform for genome engineering. However, the genome-editing activity of TnpBs remains limited and its underlying determinants are poorly understood. Here, we used biochemical and single-molecule assays to examine the DNA-unwinding mechanism of

Identifiers

PMID41542533
PMCPMC12803034

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.