Evidence map›Paper›PMID 41541500›Full record

Articlenpj women's health2025

Common cervicovaginal sequencing methods result in discordant molecular diagnoses of bacterial vaginosis and reveal strain level effects of

Courtney A Broedlow, Erik Swanson, Emily M Cherenack, Chris Basting, Nicholas Fonseca Nogueira, Michael France, Pan Yue, Ashma Chakrawarti, Ana Salazar, Lunnarie Acosta and 7 more

Abstract read
In one paragraph

Article in npj women's health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Association BetweenMicroorganisms · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Courtney A BroedlowUniversity of Minnesota School of medicine, Department of Surgery, Division of Surgical Outcomes and Precision Medicine Research, Minneapolis, MN, USA.
Erik SwansonUniversity of Minnesota School of medicine, Department of Surgery, Division of Surgical Outcomes and Precision Medicine Research, Minneapolis, MN, USA.
Emily M CherenackUniversity of Miami Miller School of Medicine, Department of Public Health Sciences, Miami, FL, USA.
Chris BastingUniversity of Minnesota School of medicine, Department of Surgery, Division of Surgical Outcomes and Precision Medicine Research, Minneapolis, MN, USA.
Nicholas Fonseca NogueiraUniversity of Miami Miller School of Medicine, Department of Medicine, Division of Infectious Diseases, Miami, FL, USA.
Michael FranceUniversity of Maryland School of Medicine, Institute for Genome Sciences, Baltimore, MD, USA.
Pan YueUniversity of Miami Miller School of Medicine, Department of Public Health Sciences, Miami, FL, USA.
Ashma ChakrawartiUniversity of Minnesota School of medicine, Department of Surgery, Division of Surgical Outcomes and Precision Medicine Research, Minneapolis, MN, USA.
Ana SalazarUniversity of Miami Miller School of Medicine, Department of Medicine, Division of Infectious Diseases, Miami, FL, USA.
Lunnarie AcostaUniversity of Miami Miller School of Medicine, Department of Medicine, Division of Infectious Diseases, Miami, FL, USA.
Patricia RaccamarichUniversity of Miami Miller School of Medicine, Department of Medicine, Division of Infectious Diseases, Miami, FL, USA.
Michael GaleUniversity of Washington, Department of Immunology, Seattle, WA, USA.
Jacques RavelUniversity of Maryland School of Medicine, Institute for Genome Sciences, Baltimore, MD, USA.
Lydia A FeinUniversity of Miami Miller School of Medicine, Department of Obstetrics, Gynecology, and Reproductive Sciences, Miami, Florida, USA.
Johanna HolmUniversity of Maryland School of Medicine, Institute for Genome Sciences, Baltimore, MD, USA.
Maria L AlcaideUniversity of Miami Miller School of Medicine, Department of Public Health Sciences, Miami, FL, USA.
Nichole R KlattUniversity of Minnesota School of medicine, Department of Surgery, Division of Surgical Outcomes and Precision Medicine Research, Minneapolis, MN, USA.

Funding

University of Miami Developmental Center for AIDS Research (D-CFAR)P30AI073961 · NIAID · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI Savita Pahwa · 2007 to 2026
$33.8M
Mechanisms underlying recurrent bacterial vaginosis and HIV risk A Multidisciplinary approachR01AI138718 · NIAID · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI ALCAIDE, MARIA LUISA · 2018 to 2023
$3.5M
NIAID NIH HHS P30 AI073961NIAID NIH HHS R01 AI138718
6 · The paper itself

Abstract

Bacterial vaginosis (BV) is associated with HIV transmission and pre-term birth, yet the etiology of BV remains unknown. Our analysis addressed that knowledge gap by comparing diagnostic techniques and using Bayesian inference to find species-specific associations with clinical indicators. We also assessed the effect of sequencing methodology on the results of molecular BV profiling. We observed significant differences in microbial diversity within BV-associated CSTs based on clinical diagnosis. CST assignments were substantially influenced by sequencing methodology, with concordance between methods as low as 59% for metatranscriptomic and metataxonomic-based CST assignment. We also found that

Indexed as

AmselBacterial vaginosisGardnerellaGardnerella vaginalisLactobacillusmetagenomemetatranscriptomeMicrobiomeNugent

Identifiers

PMID41541500
PMCPMC12803708

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.