Biofilm Formation and Antibiotic Resistance in Orthopaedic Implant Infections: A Molecular Analysis of icaA, icaD, and mecA Genes in an Indian Cohort.

Sheetal Verma, Asma Khan, Vimala Venkatesh, Shah Waliullah, Deepak Kumar, Rashmi

Abstract read

In one paragraph

Article in Indian journal of orthopaedics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Sheetal VermaDepartment of Microbiology, King George's Medical University, Chowk, 226003 Lucknow, U.P. India.

Asma KhanDepartment of Microbiology, King George's Medical University, Chowk, 226003 Lucknow, U.P. India.

Vimala VenkateshDepartment of Microbiology, King George's Medical University, Chowk, 226003 Lucknow, U.P. India.

Shah WaliullahDepartment of Orthopaedic Surgery, King George's Medical University, Chowk, Lucknow, U.P. 226003 India.

Deepak KumarDepartment of Orthopaedic Surgery, King George's Medical University, Chowk, Lucknow, U.P. 226003 India.

RashmiDepartment of Microbiology, King George's Medical University, Chowk, 226003 Lucknow, U.P. India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Orthopaedic implant-related infections (OIRIs) are a major clinical challenge, contributing to increased morbidity, prolonged hospitalisation, and higher healthcare costs. This study investigated the prevalence of biofilm-associated genes ( Methods: This was a cross-sectional study of 200 clinical samples from patients with suspected OIRIs. Pathogens were identified using MALDI-TOF MS, and antimicrobial susceptibility was tested according to CLSI 2023 guidelines. Biofilm formation was assessed by a modified microtiter plate assay, and Results: The mean patient age was 37.99 ± 18.17 years, with males comprising 74%. Fractures were the leading cause of OIRIs (72.5%), predominantly affecting the lower limb (62.5%). Conclusions: OIRIs are strongly influenced by biofilm formation and methicillin resistance. Management should prioritize biofilm-targeted therapies, precision antibiotic use, and implant surface innovations to reduce infection risk and improve outcomes.

Indexed as

Antimicrobial resistanceBiofilmicaAicaDImplant materialsInfection controlMethicillin resistanceMultidrug-resistant pathogensOrthopaedic implant infectionsStaphylococcus epidermidis

Identifiers

PMID41541020

DOI10.1007/s43465-025-01594-0

PMCPMC12799882

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.