Evidence map›Paper›PMID 41540982›Full record

ArticleAerosol and air quality research2024

Assessment of a membrane filter coated with hygroscopic glycerol for improved recovery of airborne viable bacteriophage MS2.

Mohammad Washeem, William B Vass, Drew W Becker, Amin Shirkhani, Sripriya Nannu Shankar, Yuetong Zhang, Morteza Alipanah, Z Hugh Fan, John A Lednicky, Chang-Yu Wu

Abstract read
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In one paragraph

Article in Aerosol and air quality research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mohammad WasheemDepartment of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
William B VassDepartment of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
Drew W BeckerDepartment of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
Amin ShirkhaniDepartment of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
Sripriya Nannu ShankarDepartment of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
Yuetong ZhangDepartment of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
Morteza AlipanahDepartment of Mechanical and Aerospace Engineering, University of Florida, USA.
Z Hugh FanDepartment of Mechanical and Aerospace Engineering, University of Florida, USA.
John A LednickyDepartment of Environmental and Global Health, University of Florida, USA.
Chang-Yu WuDepartment of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.

Funding

Using social networks to map and evaluate team science across CTSA hubsUL1TR001427 · NCATS · UNIVERSITY OF FLORIDA · PI MITCHELL, DUANE A. · 2015 to 2024
$37.2M
Together: Transforming and Translating Discovery to Improve HealthTL1TR001428 · NCATS · UNIVERSITY OF FLORIDA · PI MCCORMACK, WAYNE T. · 2015 to 2023
$3.9M
Multiplexed Airborne Virus Collection and Detection at the Point-of-CareR01AI158868 · NIAID · UNIVERSITY OF FLORIDA · PI FAN, Z. HUGH · 2021 to 2025
$1.6M
NCATS NIH HHS TL1 TR001428NCATS NIH HHS UL1 TR001427NIAID NIH HHS R01 AI158868NIOSH CDC HHS R21 OH012114
6 · The paper itself

Abstract

The viability of viruses collected on membrane filters during air sampling is compromised by desiccation. This leads to an underestimation of the concentration of viable viruses in the air. We investigated whether adding a hygroscopic glycerol coating to membrane filters could enhance the recovery of viable bacteriophage MS2 collected through air sampling. Mixed cellulose ester (MCE) filters were coated with glycerol and compared with as-packaged filters (APF) as a control to determine the impact of glycerol on MS2 viability. First, MS2 viability upon direct deposition of MS2 suspension on APF vs. glycerol-coated filters (GCF) was assessed at benchtop. On average 121% more viable MS2 were recovered from GCF than APF (p = 0.031). MS2 was then aerosolized and collected onto APF, GCF, phosphate-buffered saline (PBS)-wetted filters (PWF), and gelatin filters (GF). All filter types collected statistically similar (p > 0.05) amounts of aerosolized virus at 50% RH according to RT-qPCR analysis. Viable virus recovery from GCF was significantly lower than from APF (p = 0.011) at 50% RH but significantly higher (p = 0.0004) at 80% RH. APF and GCF preconditioned for 30 minutes at 80% RH were also assessed for MS2 aerosol sampling. Results showed higher viable virus recovery from GCF than either APF (p = 0.021) or GF (p = 0.006). High RH in aerosols facilitated viability conservation of MS2 on GCF, leading to improved recovery of viable virus from membrane filters.

Indexed as

BioaerosolDesiccationFiltrationHygroscopicityRelative Humidity

Identifiers

PMID41540982
PMCPMC12803739

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.