Evidence map›Paper›PMID 41539857›Full record

ArticleJournal of microbiology and biotechnology2026

Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of Live

Minkyu Park, Changwoo Park, Seung Bum Kim, Seil Kim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. DecipheringMicroorganisms · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Minkyu ParkBiometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Changwoo ParkBiometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.
Seung Bum KimDepartment of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea.
Seil KimBiometrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viability PCR (v-PCR) was optimized using propidium monoazide (PMA) and Triton X-100 for the selective detection of live foodborne pathogens in this study. The colony-forming unit (CFU) method, conventionally used for detecting live foodborne pathogens, is time-consuming. Quantitative PCR (qPCR) and droplet digital PCR (ddPCR) have emerged as rapid alternatives for pathogen detection, which provide accurate detection at low bacterial concentrations. However, these methods cannot distinguish between live and dead bacteria. We investigated the optimization of v-PCR conditions using PMA concentrations (10-200 μM) and Triton X-100 concentrations (0-1%) for

Indexed as

Cronobacter sakazakiiMicrobial ViabilityOctoxynolPolymerase Chain ReactionSalmonella entericaAzidesColony Count, MicrobialFood MicrobiologyPropidiumAzidesOctoxynolPropidiumpropidium monoazideDroplet digital PCRFoodborne diseasesPMAReal-time polymerase chain reactionTriton X-100

Identifiers

PMID41539857
PMCPMC12828127

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.