ArticleiScience2026
Recruitment of transcriptional effectors by Cas9 creates
Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Long-range regulation of transcription scales with genomic distance in a gene-specific manner.Molecular cell · 2025Article
- What is an enhancer?BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Review
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of cardiac gene expression, especially for sarcomeric genes, is crucial for heart function and is often mediated by distal regulatory elements such as enhancers and repressors. Despite their importance, the link between transcription factor recruitment to distant genomic regions and their ability to modulate gene expression remains unclear. Here, we used dead Cas9 to target either viral or endogenous cardiac transcription factor domains to naive genomic sites lacking chromatin accessibility or active regulatory marks. Remarkably, these sites underwent epigenetic remodeling, altering both local and distal promoter chromatin and significantly changing gene expression, even across insulating loci. The degree of transcriptional activation or repression varied non-linearly with the distance between the regulatory site and the gene. These findings broaden the traditional framework of enhancer and repressor function, revealing that virtually any DNA site can become regulatory, and provide fundamental insights into the rules governing gene expression in the heart.
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Registered trials
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