ArticleJournal of advanced research2026
Time-series transcriptome and metabolome profiling Uncovers WRKY6 and WRKY23 as critical regulators in tobacco response to Pseudomonas syringae infection.
Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Spatiotemporal metabolomics of tobacco leaves in response to Pseudomonas syringae infection.Plant physiology · 2026Article
- Metabolomic and Transcriptomic Responses ofPlants (Basel, Switzerland) · 2026Article
- Analysis of Metabolic Differences and Core Regulatory Pathways in Lactic Acid Bacteria-Fermented Broths of DifferentFoods (Basel, Switzerland) · 2026Article
- Temporal dynamics of photosynthetic inhibition, antioxidant defense, and transcriptomic reprogramming in 'Crimson Seedless' grapevine under saline-alkali stress.Frontiers in plant science · 2026Article
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Authors and funding
12 authors.
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Abstract
introductionPseudomonas syringae pv. tabaci, a Gram-negative bacterial pathogen, causes devastating tobacco wildfire disease with global economic impacts. While its pathogenicity is well documented, the dynamic defense mechanisms of tobacco against infection remain poorly understood.
objectiveThis study aimed to decipher phased defense mechanisms of tobacco against P. syringae infection through multi-omics integration, with emphasis on elucidating spatiotemporal coordination between transcriptional reprogramming and metabolic remodeling, and functionally validating critical regulatory modules.
methodsTime-series transcriptomic and metabolomic profiling was integrated to reconstruct dynamic response patterns. Stage-specific regulatory modules were explored via TO-GCN and WGCNA, and the roles of WRKY6 and WRKY23 in disease resistance were validated by generating transgenic lines.
resultsEarly infection (12-24 hpi) prioritized stress signaling and hormone pathway activation (salicylic acid/jasmonate), transitioning to cellular homeostasis regulation at late stages (48-60 hpi). WRKY, ERF, and NAC families orchestrated stage-specific gene expression. Notably, WRKY6 and WRKY23 functioned as negative regulators, with their silencing leading to a reduction in lesion area by 42-58% and pathogen load by 3.2-4.5 fold. Metabolomic analysis revealed sustained activation of phenylpropanoid metabolism, specifically regulating L-phenylalanine homeostasis and biosynthesis of its defense derivative xanthosine. Additionally, core modules involved in sphingolipid metabolism, light responses, and hormone cross-talk were also identified.
conclusionWe demonstrate that WRKY-mediated transcriptional reprogramming coordinates phytohormone signaling, sphingolipid dynamics, and light responses to spatiotemporally regulate secondary metabolite production. The identified WRKY6 and WRKY23 regulatory module establishes a molecular framework for engineering disease resistance, and the proposed two-phase defense model (early signaling → late metabolic remodeling) advances understanding of plant-pathogen interactions and offers targets for precision breeding.
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