Evidence map›Paper›PMID 41532838›Full record

ArticleBiotechnology journal2026

Engineering of High-Yield Recombinant Adeno-Associated Virus Producer Plasmids.

Marco T Radukic, Dinh To Le, Robert Freudenberg, Anne Hammann, Omar Hamdan, Claire Rothschild-Gronau, Raimund Hoffrogge, Susanne K Golm, Rebecca C Feiner, Kathrin E Teschner and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Marco T RadukicCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Dinh To LeCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Robert FreudenbergCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Anne HammannCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Omar HamdanCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Claire Rothschild-GronauCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Raimund HoffroggeCenter for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
Susanne K GolmCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Rebecca C FeinerCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Kathrin E TeschnerCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.
Kristian M MüllerCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Bielefeld, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recombinant Aadeno-associated virus (rAAV) production lags demand with respect to quality and quantity. We report insights from producer plasmid engineering aimed at increasing yield and homogeneity of rAAV vectors obtained by HEK-293 triple transfection. Miniaturized production and same-day quantification streamlined the investigation. We demonstrate that modifications of the AAV2 Rep gene cluster reduces titers of currently circulating packaging plasmids. Revertants to wild type yielded 116-fold higher titers of about 10

Indexed as

DependovirusGenetic EngineeringPlasmidsGenetic VectorsHEK293 CellsHumansTransfectionViral ProteinsViral ProteinsAd5 helper plasmidadeno‐associated virus (AAV)membrane‐associated accessory protein (MAAP)plasmid engineeringreplicase (Rep)

Identifiers

PMID41532838
PMCPMC12802539

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.