ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
A Mini-Spidroin Forms High-Performance Artificial Spider Silk via Edge-Cysteine-Locked β-Sheet Assembly.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Liquid-liquid phase separation enables chromatography-free purification and high-performance spidroin-amyloid hybrid silk fibers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Review
- A Mini-Spidroin Forms High-Performance Artificial Spider Silk via Edge-Cysteine-Locked β-Sheet Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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Authors and funding
11 authors.
Funding
Abstract
Spider silk's remarkable mechanical properties arise from its hierarchical organization, where β-sheet nanocrystals confer strength and amorphous chains impart extensibility. However, replicating this performance in synthetic fibers has been hindered by the challenges of expressing high-molecular-weight spidroins and processing them into fibers. Here, we overcome these limitations by engineering a mini-spidroin (∼33 kDa) that is both easily expressible and spinnable, yet yields fibers with exceptional strength and toughness. Our strategy introduces cysteine residues at the termini of polyalanine (polyA) segments, enabling inter-strand disulfide bonds that enhance molecular cohesion during liquid-liquid phase separation (LLPS). This "edge-cysteine locking" promotes directional β-sheet assembly under extensional flow, resulting in fibers with an ultimate tensile strength of 531 ± 33 MPa and toughness of 182 ± 6 MJ/m
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