ArticleAnalytical and bioanalytical chemistry2026
Comparison of light scattering-based detection methods for the sizing and number density characterization of extracellular vesicles (EV) isolated from human embryonic kidney (HEK) cell cultures.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- A Rapid, High-Resolution Chromatographic Method for Isolating Subpopulations of HEK293-derived Extracellular Vesicles.Nanotheranostics · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Extracellular vesicles (EVs) are membrane-bound nanosized particles excreted by all cells and are of significant interest for biomedical applications such as diagnostic testing and as vectors for therapeutic delivery. EVs are abundant in biofluids, including urine, saliva, blood, and cell culture media, but must be isolated from their complex matrix for use. Once isolated, a primary challenge is determining the EV size distribution and the total number of particles recovered. Multiple detection methods are currently used to characterize EV recoveries in terms of sizing and number density determinations, the most common being nanoparticle tracking analysis (NTA), flow cytometry, and electron microscopy (transmission (TEM) or scanning (SEM)). Addressed here is a practical assessment of three common light scattering-based methods for the determination of EV population sizing and number densities. NTA, multi-angle light scattering (MALS), and nano-flow cytometry (nFCM) are directly compared. Specifically, the baseline practical advantages and disadvantages of each technique are evaluated via analysis of silica nanoparticle standards. Subsequently, EVs isolated from human embryonic kidney (HEK) cell culture supernatant using a hydrophobic interaction chromatography-based separation on the previously developed polyester (PET) capillary-channeled polymer (C-CP) fiber column platform were characterized. This isolation of high-purity EVs from HEK cell culture matrix components was validated using UV chromatograms, Bradford protein assays, SEM, TEM, and fluorescence nFCM analysis. The relative attributes of these important light scattering methods are presented in terms of their fitness for specific applications and overall effectiveness in EV size and concentration analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.