Evidence map›Paper›PMID 41529903›Full record

ArticleACS synthetic biology2026

SHARK: A Specialized Host for Assembling R6K Plasmids.

Shivang Hina-Nilesh Joshi, Christopher Jenkins, David Ulaeto, Thomas E Gorochowski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shivang Hina-Nilesh JoshiSchool of Biological Sciences, University of Bristol, 24 Tyndall Avenue, Bristol BS8 1TQ, U.K.
Christopher JenkinsDefence Science and Technology Laboratory, Porton Down, Wiltshire SP4 0JQ, U.K.
David UlaetoDefence Science and Technology Laboratory, Porton Down, Wiltshire SP4 0JQ, U.K.
Thomas E GorochowskiSchool of Biological Sciences, University of Bristol, 24 Tyndall Avenue, Bristol BS8 1TQ, U.K.ORCID 0000-0003-1702-786X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

R6K plasmids are commonly used for a wide range of genome engineering applications due to their ability to support transient delivery of genetic cargos in many hosts. The maintenance of R6K plasmids requires specific strains. Unfortunately, many of these have obscure backgrounds, limited availability and were not built for efficient cloning. To address this issue, we present the construction and characterization of a series of Pir

Indexed as

Escherichia coliPlasmidsCloning, MolecularExtrachromosomal DNAGenetic EngineeringExtrachromosomal DNAcloningconditional replicationgenome integrationlambda-REDPir strainsR6K plasmids

Identifiers

PMID41529903
PMCPMC12930497

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.