Evidence map›Paper›PMID 41528713›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Complete Genome Sequence of Phytoplasma Obtained by Nanopore Sequencing Using High Molecular Weight DNA.

Yi-Ching Chiu, Helen Mae Mejia, Chih-Horng Kuo, Jun-Yi Yang

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yi-Ching ChiuInstitute of Biochemistry, National Chung Hsing University, Taichung, Taiwan.
Helen Mae MejiaInstitute of Biochemistry, National Chung Hsing University, Taichung, Taiwan.
Chih-Horng KuoDoctoral Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taichung, Taiwan. chk@gate.sinica.edu.tw.
Jun-Yi YangInstitute of Biochemistry, National Chung Hsing University, Taichung, Taiwan. jyang@nchu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nanopore sequencing can obtain long reads, which help to overcome the challenges posed by AT- and repeat-rich genomes, such as those found in phytoplasmas. Additionally, removing short DNA fragments improves the read length distribution before sequencing. Here, we present a methodology for extracting genomic DNA from phytoplasma-infected plants, the removal of short DNA fragments under 10 kb, and utilizing nanopore sequencing to generate lengthy reads. Furthermore, we outline procedures for assembling a circular genome by removing the plant sequence and refining it using short reads generated by Illumina sequencing to correct sequencing errors.

Indexed as

DNA, BacterialGenome, BacterialNanopore SequencingPhytoplasmaWhole Genome SequencingHigh-Throughput Nucleotide SequencingMolecular WeightSequence Analysis, DNADNA, BacterialIlluminaNanoporePhytoplasmaSRE

Identifiers

PMID41528713

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.