Evidence map›Paper›PMID 41528701›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

On-Site Universal Phytoplasma Detection by 23S rRNA-Gene-Targeted Loop-Mediated Isothermal Amplification (LAMP).

Mako Akahori, Akio Miyazaki, Hiroaki Koinuma, Nozomu Iwabuchi, Kensaku Maejima, Shigetou Namba, Yasuyuki Yamaji

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mako AkahoriDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Akio MiyazakiDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Hiroaki KoinumaDepartment of Agriculture, School of Agriculture, Tokai University, Kumamoto, Japan.
Nozomu IwabuchiDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Kensaku MaejimaDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. amaejima@mail.ecc.u-tokyo.ac.jp.
Shigetou NambaDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Yasuyuki YamajiDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter describes a genus-universal, specific, sensitive, rapid, and simple detection system for phytoplasmas using universal loop-mediated isothermal amplification (LAMP) primers targeting the 23S rRNA gene. Diagnosis with this system consists of a simple boiling DNA extraction method (95 °C, 10 min) and DNA amplification by the LAMP assay (64 °C, 30-60 min), with a total time of about 60 min. This system enables on-site diagnosis, as it proceeds at a constant temperature, is easy to operate, requires no experimental devices or electricity, and generates little experimental waste. Moreover, the simple boiling DNA extraction method can be applied to a variety of sample types, including herbaceous plants, woody plants, and insect vectors.

Indexed as

Molecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPhytoplasmaRNA, Ribosomal, 23SAnimalsDNA, BacterialDNA PrimersPlant DiseasesDNA, BacterialDNA PrimersRNA, Ribosomal, 23SCTABInsect vectorLoop-mediated isothermal amplification (LAMP)On-site detectionPhytoplasmaSimple DNA extractionUniversal detection

Identifiers

PMID41528701

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.