Evidence map›Paper›PMID 41528597›Full record

ArticleActa parasitologica2026

Comparative Diagnostic Efficacy of Microscopy, LAMP and PCR for Detection of Bovine Babesiosis in New Valley Governorate, Egypt.

Khatib H Abdelwahab, Mohammed E M Tolba, W Senosy, Ahmed M Ahmed, Abeer A Khedr, Khaled Abdelaziz, Shawky M Aboelhadid, Wafaa G Mahmoud

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Khatib H AbdelwahabDepartment of Parasitology, Faculty of Veterinary Medicine, New Valley University, New Valley, El-Khargah, 72511, Egypt.
Mohammed E M TolbaDepartment of Clinical Microbiology and Parasitology, College of Medicine, King Khalid University, Abha, Saudi Arabia.
W SenosyDepartment of Theriogenology, Faculty of Veterinary Medicine, New Valley University, El- Kharga, 72511, Egypt.
Ahmed M AhmedDepartment of Animal Medicine (infectious diseases), Faculty of Veterinary Medicine, New Valley University, El-Khargah, 72511, Egypt.
Abeer A KhedrDepartment of Parasitology, Faculty of Veterinary Medicine, New Valley University, New Valley, El-Khargah, 72511, Egypt.
Khaled AbdelazizDepartment of Animal and Veterinary Science, Clemson University, Clemson, SC, 29634, USA.
Shawky M AboelhadidDepartment of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni- Suef, 52611, Egypt. shawky.abohadid@vet.bsu.edu.eg.ORCID http://orcid.org/0000-0002-1403-2527
Wafaa G MahmoudDepartment of Parasitology, Faculty of Veterinary Medicine, New Valley University, New Valley, El-Khargah, 72511, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeBovine babesiosis poses a serious threat to cattle in tropical and subtropical regions. This study compared 3 methods to detect babesiosis in naturally infected cattle; microscopy, loop-mediated isothermal amplification (LAMP), and conventional PCR (cPCR), in Egypt's New Valley Governorate.

methodsA total of 339 tick-infested cattle were examined for Babesia infection. Microscopic examination was done. Genomic DNA extraction for the LAMP and PCR assays was carried out. LAMP was performed and compared with cPCR.

resultsMolecular methods revealed higher infection rates than microscopic examination: LAMP detected Babesia DNA in 40.7% (138/339) of samples, cPCR in 41.9% (142/339), while microscopy identified 31.9% (108/339). Clinically infected cattle exhibited fever, hemoglobinuria, pallor, and weight loss. Seasonal variation showed peak prevalence in summer (37.8%) and the lowest in winter (20%). Based on comparative analysis, LAMP showed 97.18% sensitivity, 100% specificity, and almost perfect agreement with cPCR (κ = 0.98, P = 0.13).

conclusionsThese findings demonstrate high Babesia prevalence in tick-infested cattle and underscore LAMP's value as a rapid, sensitive, and field-friendly tool for surveillance.

Indexed as

BabesiaBabesiosisCattle DiseasesMicroscopyMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPolymerase Chain ReactionAnimalsCattleDNA, ProtozoanEgyptSensitivity and SpecificityDNA, ProtozoanBabesiaBovine babesiosisCattleLAMPPCR

Identifiers

PMID41528597
PMCPMC12799746

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.