ArticleBMC genomics2026
Integrated analysis of DNA methylation and gene expression in dairy cows during early pregnancy.
Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundThis study investigated DNA methylation-related changes in cows during early pregnancy and constructed gene pools associated with pregnancy.
methodsWe used peripheral blood samples from six healthy, productive, and similar cows 30 days post-insemination (Pre 30) and six non-pregnant cows (Ctrl), and performed association analysis using Reduced Representation Bisulfite Sequencing (RRBS) and transcriptomic sequencing methods. Targeted bisulfite sequencing (TBS) and quantitative real-time PCR (qRT-PCR) methods were used to identify differentially methylated genes.
resultsAn obvious trend of hypomethylation was observed within the whole genome of Pre 30 dairy cows. A total of 76,530 differentially methylated sites (DMCs) were identified, comprising 32,886 hypermethylated sites and 43,644 hypomethylated sites. Additionally, there were 16,411 differentially methylated regions (DMRs), including 8,455 hypomethylated regions and 7,956 hypermethylated regions. RNA-Seq identified 44 significantly differentially expressed genes (DEGs), including 25 up and 19 down-regulated DEGs. Gene ontology (GO) analysis revealed significant enrichment in the developmental process, cell differentiation, and nervous system development in biological processes; Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated significant enrichment in cancer-related pathways, such as cancer overview and cellular and gastric cancer and immune disease signaling pathways. We validated the methylation patterns of 17 genes and showed that the overall trend was hypomethylation, consistent with sequencing results of RRBS. qRT-PCR showed that HBB, HBA1 and GPR4 were significantly increased in the Pre 30 group (P < 0.05), whereas DOK7 and ISG15 were significantly decreased (P < 0.05), consistent with transcriptome sequencing.
conclusionsOur data encompass a diverse range of transcriptional dynamics and DNA methylation alterations observed during the early stages of pregnancy in dairy cows, offering novel insights for subsequent investigations into epigenetic changes occurring during this critical period.
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