Evidence map›Paper›PMID 41526490›Full record

ArticleScientific reports2026

Utility of fluorescamine for spectrofluorimetric quantitation of berotralstat hydrochloride in spiked human plasma and content uniformity test and evaluation of method greenness.

Hesham Salem, Mohamed Gamal Eldeen, Basmala Sayed, Menna Kamel, Mostafa Embarak, Salma Mokhtar, Hoda Madian, Basmala Bahaa, Sama Alnaser, Mahmoud Abdelgaleel

Abstract read
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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hesham SalemPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt. h_salem_eg@yahoo.com.
Mohamed Gamal EldeenPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Basmala SayedPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Menna KamelPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Mostafa EmbarakPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Salma MokhtarPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Hoda MadianPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Basmala BahaaPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Sama AlnaserPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.
Mahmoud AbdelgaleelPharmaceutical chemistry department, faculty of pharmacy, Deraya University, New Minia, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel, simple and highly sensitive spectrofluorimetric approach has been developed and validated for the first time for determination of berotralstat through derivatization reaction using fluorescamine as a fluorescent probe. The reaction was carried out in buffered medium at pH 7.5, using a borate buffer. After being illuminated at 390 nm, the fluorescence intensity of the resulting product was measured at 480 nm. The proposed approach demonstrated linearity within the concentration range of 100 to 1000 ng mL

Indexed as

BenzophenoneidumFluorescamineFluorescent DyesHumansHydrogen-Ion ConcentrationLimit of DetectionReproducibility of ResultsSpectrometry, FluorescenceBenzophenoneidumFluorescamineFluorescent DyesBerotralstat hydrochlorideContent uniformity testingFluorescamineGreennessSpectrofluorimetry

Identifiers

PMID41526490
PMCPMC12800129

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.