Evidence map›Paper›PMID 41524822›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2026

Antimicrobial activity of brown propolis ethanolic extract and its application in intramammary formulation for the treatment of bovine mastitis.

Valdenice Félix da Silva, Danillo Sales Rosa, Priscila Izidro de Figueirêdo, Tania Maria Sarmento da Silva, Rodolfo de Moraes Peixoto, Mateus Matiuzzi da Costa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Valdenice Félix da Silva1Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, 64770-000, Brazil.ORCID http://orcid.org/0000-0002-9956-3828
Danillo Sales RosaUniversidade Federal Rural de Pernambuco (UFRPE), Recife, Pernambuco, 52171-900, Brazil.ORCID http://orcid.org/0000-0002-0447-5041
Priscila Izidro de FigueirêdoUniversidade Federal Rural de Pernambuco (UFRPE), Recife, Pernambuco, 52171-900, Brazil.ORCID http://orcid.org/0000-0002-7898-8077
Tania Maria Sarmento da SilvaUniversidade Federal Rural de Pernambuco (UFRPE), Recife, Pernambuco, 52171-900, Brazil.ORCID http://orcid.org/0000-0003-1887-2598
Rodolfo de Moraes Peixoto1Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, 64770-000, Brazil.ORCID http://orcid.org/0000-0002-0438-094X
Mateus Matiuzzi da Costa1Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, 64770-000, Brazil. mmatiuzzicosta@gmail.com.ORCID http://orcid.org/0000-0002-9884-2112

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico Conselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior Finance Code 001Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco BFP-0368-5.05/24Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco IBPG-1650-2.00/21
6 · The paper itself

Abstract

The major sanitary concern in dairy cattle production chain is mastitis, causing uncountable losses, which implies in the need of alternative therapies. Thus, the purpose of this study was to evaluate the antimicrobial activity of brown propolis ethanolic extract and its therapeutic potential for application in bovine intramammary mastitis therapeutic formulation. In the in vitro study we analyzed 40 Staphylococcus sp. isolates from subclinical mastitis cases. The chemical profile of extract was obtained by High Performance Liquid Chromatography. The antimicrobial activity was determined by broth microdilution. The biofilm formation was verified in the microplate adherence assay. To test the in vitro antimicrobial potential of the formulation, 28 bovine mammary glands were used. Mastitis was confirmed by bacterial culture, somatic cells counts (SCC) and California Mastitis Test on seven experimental occasions. In the extract, 28 flavonoids were identified, and this showed antimicrobial activity against all isolates tested in a range between 781 and 1,563 µg ml

Indexed as

Anti-Bacterial AgentsAnti-Infective AgentsMastitis, BovinePropolisStaphylococcal InfectionsStaphylococcusAnimalsBiofilmsCattleEthanolFemaleMammary Glands, AnimalMicrobial Sensitivity TestsAnti-Bacterial AgentsAnti-Infective AgentsEthanolPropolisDiagnosisLactoculturePhytotherapySCC

Identifiers

PMID41524822
PMCPMC12796081

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.