Evidence map›Paper›PMID 41524783›Full record

ArticleArchives of microbiology2026

Evaluation of a recombinant Loa22-gold nanoparticle based lateral flow assay for the serodiagnosis of leptospirosis in canine and bovine.

Himani Gautam, B V Sunil Kumar, Satparkash Singh, M Manu

Abstract readEvaluation Study
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Himani GautamCollege of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
B V Sunil KumarCollege of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India. drbvskumar@gmail.com.
Satparkash SinghCollege of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
M ManuCollege of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Leptospirosis is a globally significant zoonotic disease caused by the pathogenic Leptospira spp. This study aimed to develop a recombinant Loa22 based lateral flow assay for detecting leptospirosis in dogs and cattle. The loa22 gene from Leptospira interrogans was cloned, expressed in E. coli, and purified using Ni-NTA affinity chromatography. The immunoreactivity of the recombinant protein was confirmed by western blotting, and hyperimmune sera against the protein was raised in mice. Gold nanoparticles (AuNP) were conjugated with rLoa22 under optimized conditions and characterized by UV-Vis spectrophotometry, agarose gel electrophoresis, and transmission electron microscopy. The lateral flow assay (LFA) strips were assembled with AuNP-rLoa22 on the conjugate pad and rLoa22 and hyperimmune sera on the test and control lines, respectively. Diagnostic performance of the assay was evaluated using sera from 100 dogs and 102 cattle suspected for leptospirosis and compared to the reference standard microscopic agglutination test (MAT). Six dog and cattle samples each were tested positive by MAT. The developed LFA further tested 06 dog and 07 cattle samples as positive in addition to the MAT positive samples, representing false positives. In dogs, the LFA showed 100% sensitivity and 93.62% specificity, while in cattle, it showed 100% sensitivity (Se) and 92.71% specificity (Sp) with respect to MAT. The n/N for Se and Sp were, Se

Indexed as

Antigens, BacterialBacterial Outer Membrane ProteinsCattle DiseasesDog DiseasesLeptospira interrogansLeptospirosisMetal NanoparticlesSerologic TestsAnimalsAntibodies, BacterialCattleDogsGoldLeptospiraLipoproteinsRapid Diagnostic TestsAntibodies, BacterialAntigens, BacterialBacterial Outer Membrane ProteinsGoldLipL32 protein, LeptospiraLipoproteinsRecombinant ProteinsLateral flow assayLeptospiraLoa22Rapid diagnostic testRecombinant proteinZoonotic

Identifiers

PMID41524783

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.