Evidence map›Paper›PMID 41524411›Full record

ArticleApplied and environmental microbiology2026

High-density biomass as a substrate for stimulating biosulfidogenesis in the deep layer of stratified acidic pit lakes.

Yutong Liu, Rachel A Brennan, Javier Sánchez-España, Carlos Vilchez, Juan-Luis Fuentes, Jennifer L Macalady, William D Burgos

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yutong LiuDepartment of Civil and Environmental Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0009-0001-7629-5082
Rachel A BrennanDepartment of Civil and Environmental Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0000-0001-6780-7552
Javier Sánchez-EspañaDepartment of Planetology and Habitability, Centro de Astrobiología (CAB), Spanish National Research Council (CSIC), Torrejón de Ardoz, Madrid, Spain.ORCID 0000-0001-6295-1459
Carlos VilchezAlgal Biotechnology Group, CIDERTA and Faculty of Experimental Sciences, University of Huelva, Huelva, Spain.ORCID 0000-0002-8164-1672
Juan-Luis FuentesAlgal Biotechnology Group, CIDERTA and Faculty of Experimental Sciences, University of Huelva, Huelva, Spain.ORCID 0000-0002-9037-3147
Jennifer L MacaladyDepartment of Geosciences, The Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0000-0001-5214-0601
William D BurgosDepartment of Civil and Environmental Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0000-0003-3269-2921

Funding

National Science Foundation EAR-2016826
6 · The paper itself

Abstract

Accumulation of harmful metal(loid)s in acidic pit lakes (APLs) is a serious environmental issue in mining districts. This lab-based study evaluated a novel method to stimulate dissimilatory sulfate reduction to promote the formation of sparingly soluble metal(loid)-sulfide minerals in the permanently stratified deep layer of Cueva de la Mora (CM), an APL in the Iberian Pyrite Belt in Spain. Solid-phase biomass was selected because it can be pressed into high-density forms that are dense enough to settle into the deep layer of a lake. This "direct delivery" of electron donor overcomes the current "indirect method" to stimulate algae growth in the upper layer and wait for algae to die and settle into the deep layer. We added the microalgae

Indexed as

BiomassLakesMicroalgaeSulfatesSulfidesWater Pollutants, ChemicalBacteriaSpainSulfatesSulfidesWater Pollutants, Chemicalacidic pit lakebioremediationhigh-density biomassmetal(loid) removalsulfate reducing bacteria

Identifiers

PMID41524411
PMCPMC12915300

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.