ArticleWorld journal of experimental medicine2025
Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis.
Article in World journal of experimental medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundIndia has the highest tuberculosis (TB) burden in the world. Of the estimated annual 10 million TB cases, features of extra pulmonary TB are evident in up to 45%. Urogenital TB (UGTB) accounts for approximately 20% of those cases. The lack of non-sputum based diagnostic tools continue to hinder efforts to reduce the burden of UGTB. MicroRNAs (miRNAs) play a crucial role in biological pathways and can be used as a potential biomarker for TB. We evaluated urinary extracellular vesicles (uEVs) as non-invasive source to explore miRNAs with biomarker potential for UGTB.
aimTo evaluate the potential of miRNA-155-5p, miRNA-26a-5p and miRNA-29a-3p in uEVs to diagnose UGTB in adults.
methodsuEV characterization was done using nanoparticle tracking analysis and flow cytometry. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for urinary uEV-miRNAs were carried out in samples from patients with suspected UGTB, or Urinary tract infections [UTI, disease controls (DC)] and healthy controls (HCs) (
resultsuEVs from UGTB or UTI patients had higher mean size, and also lower proportion of CD63 positive vesicles as compared to HC's uEVs. Between UTI and UGTB, the mean size of uEVs was significantly higher in UTI cases. qRT-PCR analysis revealed a significantly lower abundance of miRNA-155-5p and miRNA-26a-5p in uEVs from UGTB relative to UTI (
conclusionThe differential expression of uEV-miRNAs, miRNA-155-5p and miRNA-29a-3p in UTGB and UTI cases hold promise in the specific diagnosis of UGTB. Further studies in large cohort are, however, needed to confirm the diagnostic accuracy of these uEV-miRNAs.
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