ArticlePlant biotechnology (Tokyo, Japan)2025
Fluorescence staining of the nucleus in living plant cells using dimidium bromide.
Article in Plant biotechnology (Tokyo, Japan), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear staining using fluorescent dyes is crucial for cytological studies in plants. However, few fluorescent dyes are suitable for live-cell imaging of the nucleus. Here, we demonstrate that dimidium bromide (DimBr), a commercially available fluorescent dye, can be used to stain the nucleus (nucleolus and nucleoplasm) in living plant cells. DimBr emits peak fluorescence at 600 nm at an excitation wavelength of 525 nm, making it well suited for use with green fluorescent protein. DimBr staining can be used in various plant species and allows time-lapse observation of the nucleus. Therefore, DimBr can be used to visualize the nucleus in living plant cells, making it a valuable tool for plant cell biology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.