ArticleAvicenna journal of medical biotechnology
The Quality and Quantity of Nanoparticles Extracted from Human Adipose Tissue Derived-Mesenchymal Stem Cells.
Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Principles and regulatory pathways of exosome-based products in wound healing: Insights from the Iranian Food and Drug Administration guideline.Iranian journal of basic medical sciences · 2026Review
- Characterization of exosomes from hypoxia-activated human amniotic membrane mesenchymal stem cells.Iranian journal of basic medical sciences · 2026Article
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Authors and funding
4 authors.
Funding
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Abstract
Background: Nanoparticles, as small extracellular vesicles, are considered promising tools in tissue engineering and regenerative medicine. This study aimed to investigate the effects of different processing and culture condition on the quality and quantity of extracts derived from human Adipose-Mesenchymal Stem Cells (AD-MSCs). Methods: AD-MSCs were proliferated in both the experimental and control groups. Nanoparticles were extracted from AD-MSCs-extracts and analyzed using SEM, TEM, DLS, Zeta potential, FTIR and BCA analyses. The morphological characteristics (shape, size, distribution, surface topography, and agglomeration/aggregation), structural appearance (poly-disperse intensity, colloidal particle behavior, surface charge, and stability), chemical properties (functional groups and ionic interactions) and total protein concentration were detected in the extracted nanoparticles. Additionally, the morphological characteristics, apoptosis, mitochondrial oxidoreductase activity, and migration potential of AD-MSCs in both groups were evaluated using acridine orange staining, MTT, and scratch assays. Results: In the experimental group, 100% of the nanoparticles had a diameter of 112.8± 25 Conclusion: Nanoparticles derived from AD-MSCs have high therapeutic applications in tissue engineering and regenerative medicine.
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