ArticleOrthopaedic journal of sports medicine2026
Combination of Atelocollagen Gel With Induced Pluripotent Stem Cell-Derived Tenocyte for Rotator Cuff Tendon Regeneration in a Rat Model.
Article in Orthopaedic journal of sports medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Despite successful mechanical rotator cuff repair, the intrinsic quality of the tendon often remains poor, thereby increasing the risk of retear. Purpose: To investigate a proper differentiation of tenocytes from induced pluripotent stem cells (iPSCs) and a regenerative approach using iPSC-derived tenocytes (iPSC-TCs) combined with atelocollagen gel to enhance tendon healing after rotator cuff repair. Study Design: Controlled laboratory study. Methods: Tenogenic differentiation was induced in vitro from the human iPSC line 1231A3, with differentiation confirmed by cell morphology, flow cytometry, and immunofluorescence staining. The viability of iPSC-TC within 3% atelocollagen gel was analyzed. The Sprague Dawley rats were divided into 4 groups (n = 16 per group). Group 1 acted as the control without damage; group 2 with surgical repair of the fully torn supraspinatus; group 3 with additional injection of atelocollagen after the repair; and group 4 with additional injection of iPSC-TC embedded on atelocollagen after the repair. After tissue harvest at postoperative 6 weeks, quantitative evaluation of gene expression (messenger ribonucleic acid levels), immunohistochemistry (percentage of stained area), and Western blot (relative protein expression) was performed. All experimental results are reported as mean and error, and analyzed using the Student Results: Differentiation to iPSC-TC was confirmed on day 19 of passage 3 with positive expression of cell surface markers and positive expression of tenogenic markers on immunofluorescence staining. Differentiated iPSC-TC showed significantly higher expression of tendon-related genes when compared with nondifferentiated iPSC. After the tissue harvest, PKH16-labeled iPSC-TC was identified only in group 4 under confocal microscopy. The Masson trichrome stain showed significantly greater collagen intensity in group 4 compared with the other repair groups ( Conclusion: Our study demonstrates that an injection of atelocollagen containing iPSC-TCs into the lesion after rotator cuff repair produced excellent residual effects. The addition of iPSC-TCs embedded in atelocollagen to a surgically repaired rotator cuff could improve tendon quality by increasing type I collagen production. Clinical Relevance: The combination of atelocollagen with induced pluripotent stem cell-derived tenocytes could help tendon healing and thus potentially reduce retear. This approach may have the potential to improve the biological quality of repaired rotator cuffs, reduce the risk of retear, and ultimately enhance long-term surgical outcomes in patients with tendon injuries.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.