Evidence map›Paper›PMID 41521899›Full record

ArticleThe veterinary quarterly2026

Detection of five viruses commonly implicated with bovine respiratory disease using loop-mediated isothermal amplification.

Josiah Levi Davidson, Murali Kannan Maruthumuthu, Mohamed Kamel, Suraj Mohan, Ana Pascual-Garrigos, Andres Dextre, Ruth Eunice Centeno-Delphia, Jacquelyn P Boerman, Deepti Pillai, Jennifer Koziol and 4 more

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Article in The veterinary quarterly, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Josiah Levi DavidsonDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-2271-1984
Murali Kannan MaruthumuthuDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-5262-3600
Mohamed KamelDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.
Suraj MohanDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-9634-7679
Ana Pascual-GarrigosDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.ORCID 0009-0008-9873-6070
Andres DextreDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.ORCID 0009-0007-8292-2816
Ruth Eunice Centeno-DelphiaDepartment of Animal Science, Purdue University, West Lafayette, IN, USA.ORCID 0000-0003-1250-8875
Jacquelyn P BoermanDepartment of Animal Science, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-0336-8295
Deepti PillaiDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.ORCID 0000-0003-0831-2407
Jennifer KoziolDepartment of Veterinary Clinical Science, Purdue University, West Lafayette, IN, USA.ORCID 0000-0003-0096-5040
Aaron AultDepartment of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, USA.
Jon P SchoonmakerDepartment of Animal Science, Purdue University, West Lafayette, IN, USA.ORCID 0000-0001-6641-0166
Timothy A JohnsonDepartment of Animal Science, Purdue University, West Lafayette, IN, USA.
Mohit S VermaDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.ORCID 0000-0002-6374-3333

Funding

Quality Assurance and Quality Control Project Management: Improving Submissions and Study Conduct in the Human Subjects Research Prior Approval ProcessUL1TR002529 · NCATS · INDIANA UNIVERSITY INDIANAPOLIS · PI MOE, SHARON M, WIEHE, SARAH ELIZABETH · 2018 to 2022
$27.2M
NCATS NIH HHS UL1 TR002529
6 · The paper itself

Abstract

Herein, we present novel quantitative loop-mediated isothermal amplification (qLAMP) and reverse-transcription qLAMP (RT-qLAMP) assays for the detection of five viruses implicated with the onset and progression of bovine respiratory disease (BRD): Bovine Alphaherpesvirus Type 1 (BHV-1), Bovine Adenovirus Type 3 (BAV-3), Bovine Respiratory Syncytial Virus (BRSV), Bovine Viral Diarrhea Virus Type 1 (BVDV-1), and Bovine Parainfluenza Virus Type 3 (BPIV-3). Using contrived samples spiked with whole viruses, our extraction-free assays have limits of detection between 30 and 1,057 copies per reaction (1.8% final sample concentration) with minimal sample processing. Using dual-tipped swabs and 1.4 mL resuspension volumes, limits of detection are on the order of 2 × 10

Indexed as

Bovine Respiratory Disease ComplexCattle DiseasesMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRespiratory Tract InfectionsAnimalsCattleHerpesvirus 1, BovineLimit of DetectionParainfluenza Virus 3, BovineRespiratory Syncytial Virus, BovineSensitivity and Specificityassay developmentBovine Respiratory Diseasechute-side diagnosticsextraction-free detectionLAMPminimal sample processingviral BRD

Identifiers

PMID41521899
PMCPMC12798664

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.