ArticleNucleic acids research2026
Foliar-applied double-stranded RNA is mobile, transfers to plant pathogens, and triggers RNAi.
Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Identification of broadly expressed nervous system-related genes as effective RNAi targets in the brown marmorated stink bug, Halyomorpha halys.Pest management science · 2026Article
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Forest tree-pathogen interactions under climate change.Essays in biochemistry · 2026Review
- The unfulfilled potential of nanocarriers for RNA delivery in antiviral crop protection.Nature plants · 2026Review
- Targeted Suppression of the Tomato PathogenJournal of fungi (Basel, Switzerland) · 2026Article
- Spray-induced gene silencing for plant disease control: mechanistic basis and deployment-oriented design.Frontiers in plant science · 2026Review
- Rethinking control strategies for plant-parasitic nematodes.Journal of nematology · 2026Article
- BioCNT-TLS engineered systemic mobile resistome for sustainable crop protection.Frontiers in plant science · 2026Review
- Exogenous 24nt siRNAs induce AGO4A-dependent silencing via promoter DNA methylation and H3K9me2 deposition.Frontiers in plant science · 2026Article
- Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.Current opinion in plant biology · 2023Review
- Extracellular RNA: mechanisms of secretion and potential functions.Journal of experimental botany · 2023Article
- Carbon Dots Boost dsRNA Delivery in Plants and Increase Local and Systemic siRNA Production.International journal of molecular sciences · 2022Article
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Authors and funding
8 authors.
Funding
Abstract
Foliar application of double-stranded RNA (dsRNA) as RNA interference (RNAi)-based biopesticides represents a sustainable alternative to chemical-based crop protection strategies. A key feature of RNAi in plants is its ability to act non-cell autonomously, a process that plays a critical role in plant development and protection against pathogens. Whether RNAi induced by foliar dsRNA application acts non-cell autonomously remains debated, with the mechanisms and implications of this movement largely unexplored. We show that upon foliar application, dsRNA enters the leaf vasculature and moves to vegetative, reproductive, and belowground tissues in multiple plant species. Unprocessed mobile dsRNA was detected in the apoplast, being maintained in new growth, indicating apoplastic rather than symplastic transport. Mobile dsRNA could transfer to infecting fungi, where it was processed and loaded by the fungal RNAi machinery to elicit gene silencing. Using a novel biochemical purification technique and small RNA sequencing, we detected functional small interfering RNA species derived from foliar-applied dsRNA that elicit effective silencing in both the applied and distal tissue types. Our mechanistic dissection of the uptake and movement of dsRNA provides crucial insights into the mode of action of RNAi biopesticides and stands to add significant benefit to this emerging field of plant protection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.