Evidence map›Paper›PMID 41521337›Full record

ArticleBiotechnology letters2026

Metabolic engineering of Bacillus subtilis for enhanced free heme biosynthesis by an enzyme-chassis co-optimization strategy.

Shuoqi Diao, Haoqiang Zhou, Yang Li, Jingcheng Dai, Dazhong Yan, Jing Wu

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shuoqi Diao *School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.
Haoqiang Zhou *School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.
Yang LiSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.
Jingcheng DaiSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.
Dazhong YanSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China.
Jing WuSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China. jing_wu@whpu.edu.cn.ORCID http://orcid.org/0000-0003-2328-001X

Funding

Key Research and Development Program of Hubei Province 2022BBA0040National Natural Science Foundation of China 31800111
6 · The paper itself

Abstract

Heme, an iron-incorporated porphyrin compound, serves as the prosthetic group for numerous proteins involved in diverse biological processes. The prokaryotic heme biosynthetic pathway features a complex cascade of reactions, in which glutamyl-tRNA reductase (GluTR) catalyzes the formation of 5-aminolevulinic acid (ALA) that represents a critical rate-limiting step and determines ultimate heme yield. In this study, OsGluTR

Indexed as

Aldehyde OxidoreductasesBacillus subtilisHemeMetabolic EngineeringAminolevulinic AcidBacterial ProteinsEscherichia coliMutagenesis, Site-DirectedOryzaRecombinant ProteinsAldehyde OxidoreductasesAminolevulinic AcidBacterial Proteinsglutamyl tRNA reductaseHemeRecombinant Proteins5-aminolevulinic acidBacillus subtilisGlutamyl-tRNA reductaseHeme

Identifiers

PMID41521337

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.