Evidence map›Paper›PMID 41518499›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Yeast One-Hybrid (Y1H) Assay for Single Promoter-Transcription Factor Interaction in Higher Plants.

Aileen Turner, Paz E Zuñiga, Carlos R Figueroa

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Aileen TurnerLaboratory of Plant Molecular Physiology, Institute of Biological Sciences, Campus Talca, Universidad de Talca, Talca, Chile.
Paz E ZuñigaLaboratory of Plant Molecular Physiology, Institute of Biological Sciences, Campus Talca, Universidad de Talca, Talca, Chile.
Carlos R FigueroaLaboratory of Plant Molecular Physiology, Institute of Biological Sciences, Campus Talca, Universidad de Talca, Talca, Chile. cfigueroa@utalca.cl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The yeast one-hybrid (Y1H) assay is a powerful molecular biology tool for studying protein-DNA interactions. This technique involves using a single DNA bait cloned upstream of a reporter gene in a yeast plasmid to determine whether a specific transcription factor (TF) or other DNA-binding protein (referred to as "prey") can bind to the DNA bait. Typically, this technique employs a complete cDNA library to obtain multiple prey proteins, which are then introduced into yeast cells containing the bait construct. In this protocol, we present a simplified method not centered on finding new prey from cDNA libraries but on analyzing specific target plant proteins. The protocol provides a step-by-step guide for cloning DNA-bait and protein-prey constructs, transforming yeast, and screening for reporter interactions.

Indexed as

Plant ProteinsPlantsPromoter Regions, GeneticTranscription FactorsTwo-Hybrid System TechniquesCloning, MolecularGene LibraryGenes, ReporterProtein BindingSaccharomyces cerevisiaePlant ProteinsTranscription FactorsPromoterProtein-DNA interactionsTranscription factorYeast one-hybrid

Identifiers

PMID41518499

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.