Evidence map›Paper›PMID 41517932›Full record

ArticleChembiochem : a European journal of chemical biology2026

Library-on-Library Intercellular Labeling for Selection of Biotin Ligase and Acceptor Peptides.

Benya Lakkanasirorat, Phatipon Kongkamnead, Rawiporn Amornloetwattana, Pansa Leejareon, Chayasith Uttamapinant, Wenjing Wang

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In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Benya LakkanasiroratSchool of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan, Rayong, 21210, Thailand.ORCID https://orcid.org/0000-0003-4332-055X
Phatipon KongkamneadSchool of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan, Rayong, 21210, Thailand.
Rawiporn AmornloetwattanaSchool of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan, Rayong, 21210, Thailand.
Pansa LeejareonSchool of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan, Rayong, 21210, Thailand.
Chayasith UttamapinantSchool of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan, Rayong, 21210, Thailand.ORCID https://orcid.org/0000-0003-0114-0577
Wenjing WangLife Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI, 48109, USA.ORCID https://orcid.org/0000-0001-6025-9848

Funding

National Science Research and Innovation Fund (NSRF)Research and Innovation B42G670039Sloan Research Fellowships FG-2024-22156University of Michigan, the Camille Dreyfus and Henry Foundation TC-23-084VISTEC
6 · The paper itself

Abstract

Library-on-library (LOL) selection screens combinatorial libraries to generate new protein pairs. Previously, LOL selection has only been applied to stable protein-protein interactions. To extend LOL to transient enzyme-substrate pairs, a generalizable sequential LOL trans- and cis-labeling platform is developed, and a proof-of-concept selection is performed on Escherichia coli biotin ligase (BirA) and its acceptor peptide (AP). Using yeast surface display, AP mutant libraries are selected against BirA mutant libraries to identify AP variants trans-biotinylated by BirA mutants. Matched BirA mutants are subsequently enriched via the SpyTag-SpyCatcher-mediated cis-labeling platform. This represents the first demonstration of enzyme-peptide substrate LOL selection and offers a versatile framework for engineering new enzyme-peptide substrate pairs with varied activities.

Indexed as

Carbon-Nitrogen LigasesEscherichia coli ProteinsPeptide LibraryPeptidesRepressor ProteinsBiotinEscherichia coliMutationSubstrate SpecificitySulfurtransferasesBiotinbiotin synthetasebirA protein, E coliCarbon-Nitrogen LigasesEscherichia coli ProteinsPeptide LibraryPeptidesRepressor ProteinsSulfurtransferasesacceptor peptidesbiotin ligasecis‐labelinglibrary‐on‐library selectionstrans‐labeling

Identifiers

PMID41517932
PMCPMC12789891

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.