Evidence map›Paper›PMID 41517870›Full record

ArticlePlant communications2026

Prime editing of an RR26-responsive cis-element enhances DAHPS2 expression and submergence tolerance in rice through the shikimate pathway.

Dongdong Chen, Linlin Hou, Zhennan Qiu, Qiang Xu, Qiaoyan Wang, Man Li, Zhiqi Hao, Pengfei Dong, Guangheng Zhang, Jiang Hu and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Dongdong ChenState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Linlin HouState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Zhennan QiuCollege of Life Sciences, Dezhou University, Dezhou 253023, China.
Qiang XuShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Qiaoyan WangState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Man LiState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Zhiqi HaoState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Pengfei DongState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Guangheng ZhangState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Jiang HuState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Zhenyu GaoState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Guojun DongState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Deyong RenState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Lan ShenState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Yuchun RaoCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
Qing LiState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Yuhang ZhangState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China.
Qiang ZhangState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Longbiao GuoState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Lianguang ShangShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China. Electronic address: shanglianguang@caas.cn.
Qian QianState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Yazhouwan National Laboratory, Sanya 572024, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China. Electronic address: qianqian@caas.cn.
Li ZhuState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China; Yazhouwan National Laboratory, Sanya 572024, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China. Electronic address: zhuli05@caas.cn.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Early postgermination growth is critical for uniform seedling emergence in direct-seeded rice; however, the underlying regulatory mechanisms remain unclear. Here, we identified DS1, which encodes the shikimate pathway entry enzyme DAHPS2, from a dwarf and sterile mutant (ds1) in 'Huazhan' (HZ). Loss of DS1 disrupted the shikimate pathway, reduced indole-3-acetic acid (IAA) levels through the downstream tryptophan-dependent IAA biosynthesis pathway, and induced excessive jasmonic acid (JA) accumulation, resulting in severely impaired postgermination growth. Exogenous application of the auxin analog 1-naphthaleneacetic acid or the JA biosynthesis inhibitor diethyldithiocarbamic acid partially rescued the mutant phenotype. Conversely, DS1 overexpression elevated IAA levels, reduced JA accumulation, and promoted postgermination growth, facilitating rapid seedling emergence under submergence conditions. This effect was further validated in the 'Zhongjia 3' cultivar. We demonstrate that DS1 is transcriptionally activated by RR26, a type-B cytokinin (CK) response regulator, through direct binding to the DS1-7 cis element. Using prime editing, we precisely modified DS1-7 in HZ to generate transgene-free germplasm with improved DS1 expression and enhanced submergence tolerance. Collectively, our findings establish an RR26-DS1 module that regulates IAA-JA homeostasis through the shikimate pathway, providing mechanistic insights into postgermination growth and valuable genetic resources for breeding direct-seeded rice cultivars.

Indexed as

OryzaPlant ProteinsShikimic AcidCyclopentanesGene Expression Regulation, PlantIndoleacetic AcidsOxylipinsPlants, Genetically ModifiedCyclopentanesIndoleacetic Acidsjasmonic acidOxylipinsPlant ProteinsshikimateShikimic AcidauxinDAHPS2direct-seeded ricejasmonic acidpostgermination growth

Identifiers

PMID41517870
PMCPMC13084075

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.