Evidence map›Paper›PMID 41516375›Full record

ReviewInternational journal of molecular sciences2026

2-Hydroxy-4-Methoxybenzaldehyde (2H4MB): Integrating Cell Culture, Metabolic Engineering, and Intelligent Genome Editing.

Fatima Firdaus, Vikas Yadav, Muthusamy Ramakrishnan, Adla Wasi, Irfan Bashir Ganie, Anamica Upadhyay, Anwar Shahzad, Zishan Ahmad

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fatima FirdausDepartment of Chemistry, University of Lucknow, Lucknow 226007, India.
Vikas YadavDepartment of Botany, Aligarh Muslim University, Aligarh 202002, India.
Muthusamy RamakrishnanState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Centre for Sustainable Forestry in Southern China, Bamboo Research Institute, Key Laboratory of National Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, School of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.ORCID 0000-0001-7819-339X
Adla WasiDepartment of Botany, Aligarh Muslim University, Aligarh 202002, India.
Irfan Bashir GanieDepartment of Botany, Aligarh Muslim University, Aligarh 202002, India.
Anamica UpadhyayBotany Department, School of Life Sciences, Dr. Bhimrao Ambedkar University, Agra 282007, India.
Anwar ShahzadState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Centre for Sustainable Forestry in Southern China, Bamboo Research Institute, Key Laboratory of National Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, School of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.ORCID 0000-0002-6526-9196
Zishan AhmadState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Centre for Sustainable Forestry in Southern China, Bamboo Research Institute, Key Laboratory of National Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, School of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.

Funding

Young Foreign Talent Program Y20240114
6 · The paper itself

Abstract

2-Hydroxy-4-Methoxybenzaldehyde (2H4MB) is a valuable aromatic compound with applications in flavour, fragrance, and pharmaceuticals. Because of its endangered status and root-specific accumulation, its production in native plants is restricted. In order to increase 2H4MB yield, this study emphasises recent developments in metabolic engineering, synthetic biology, in vitro culture methods, and AI-assisted route prediction. This review discussed about how CRISPR-based genome editing can be used to modify important biosynthetic genes and regulatory components, as well as how predictive machine learning techniques can be used to improve production conditions. Inadequate genetic resources, poorly understood biosynthetic pathways, and a dearth of reliable transformation systems are among the present constraints. The work highlights the importance of using integrative plant biotechnology techniques to fully realise the industrial and medicinal potential of this underutilised chemical.

Indexed as

BenzaldehydesGene EditingMetabolic EngineeringBiosynthetic PathwaysCell Culture TechniquesSynthetic BiologyBenzaldehydes2-Hydroxy-4-Methoxybenzaldehyde (2H4MB)in vitro culturemetabolic engineeringsecondary metabolitessynthetic biology

Identifiers

PMID41516375
PMCPMC12786920

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.