Evidence map›Paper›PMID 41516283›Full record

ArticleInternational journal of molecular sciences2025

Search for Potential VDR/Partner Composite Elements in Regulatory DNA of Genes Associated with Respiratory Infections and Atopic Diseases.

Alexey V Popov, Dmitry Yu Oshchepkov, Vladislav V Kononchuk, Tatiana S Kalinina, Ilya S Valembakhov, Alexander D Lukin, Elena G Kondyurina, Vera V Zelenskaya, Valentin Vavilin

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Alexey V PopovInstitute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630060, Russia.
Dmitry Yu OshchepkovInstitute of Cytology and Genetics, Novosibirsk 630090, Russia.ORCID 0000-0002-6097-5155
Vladislav V KononchukInstitute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630060, Russia.ORCID 0000-0003-4070-2421
Tatiana S KalininaInstitute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630060, Russia.ORCID 0000-0002-2698-0866
Ilya S ValembakhovInstitute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630060, Russia.ORCID 0009-0001-7727-7847
Alexander D LukinInstitute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630060, Russia.ORCID 0009-0008-0933-9971
Elena G KondyurinaDepartment of Pediatrics, Faculty of Advanced Training and Professional Retraining, Novosibirsk State Medical University, Novosibirsk 630091, Russia.
Vera V ZelenskayaDepartment of Pediatrics, Faculty of Advanced Training and Professional Retraining, Novosibirsk State Medical University, Novosibirsk 630091, Russia.
Valentin VavilinInstitute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630060, Russia.

Funding

Russian-government funding 125031203556-7
6 · The paper itself

Abstract

Vitamin D deficiency is associated with the risk of atopic diseases and respiratory infections. The activated vitamin D receptor (VDR) forms a dimer with the retinoid X receptor alpha (RXRA) and binds to VDR/RXRA composite elements (CEs) in enhancers of target genes. However, VDR/RXRA CEs are identified in only 11.5% of cases in ChIP-Seq peaks. Our hypothesis was that VDR could form a VDR-Partner complex with transcription factor for which CEs have not yet been identified. We utilized Web-MCOT to search for novel VDR/Partner CEs in regulatory DNA. The potential formation of the VDR-Partner protein complex was assessed using the AlphaFold machine learning model. Through real-time RT-PCR, we measured the expression of immune system genes in a culture of U937 macrophage-like cells incubated with the active metabolite of vitamin D, calcitriol. We have predicted novel VDR/NR2C2 and VDR/PPARG CEs in the regulatory regions of immune system genes. We found potential synergism of VDR/NR2C2 and VDR/RXRA CEs in relation to the

Indexed as

Receptors, CalcitriolRegulatory Sequences, Nucleic AcidRespiratory Tract InfectionsCalcitriolDermatitis, AtopicGene Expression RegulationHumansPPAR gammaRetinoid X Receptor alphaU937 CellsCalcitriolPPAR gammaPPARG protein, humanReceptors, CalcitriolRetinoid X Receptor alphaVDR protein, humanatopycomposite elementslung infectionNR2C2PPARGVDR

Identifiers

PMID41516283
PMCPMC12786173

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.