Evidence map›Paper›PMID 41514218›Full record

ArticleBMC genomics2026

Overexpression of ginkbilobin-2 homologous domain gene to enhance the tolerance to Phytophthora cinnamomi in plants of European chestnut.

Susana Serrazina, Mª Teresa Martínez, Silvia Valladares, Lucía Del Castillo-González, Marcelo Francisco, Marta Berrocal-Lobo, Eduardo Piñas, Pablo Piñeiro, Rui Malhó, Rita Lourenço Costa and 1 more

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Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Susana SerrazinaBioISI - Biosystems and Integrative Sciences Institute, Faculty of Sciences of the University of Lisbon, Campo Grande, Lisbon, 1749-016, Portugal.
Mª Teresa MartínezMisión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (MBG-CSIC), Avda Vigo s/n, Campus Vida, Santiago de Compostela, A Coruña, 15705, Spain.
Silvia ValladaresMisión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (MBG-CSIC), Avda Vigo s/n, Campus Vida, Santiago de Compostela, A Coruña, 15705, Spain.
Lucía Del Castillo-GonzálezCentro para la Biodiversidad y Desarrollo Sostenible (CBDS), ETSI Montes, Forestal y del Medio Natural, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, Madrid, 28040, Spain.
Marcelo FranciscoBioISI - Biosystems and Integrative Sciences Institute, Faculty of Sciences of the University of Lisbon, Campo Grande, Lisbon, 1749-016, Portugal.
Marta Berrocal-LoboCentro para la Biodiversidad y Desarrollo Sostenible (CBDS), ETSI Montes, Forestal y del Medio Natural, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, Madrid, 28040, Spain.
Eduardo PiñasMisión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (MBG-CSIC), Avda Vigo s/n, Campus Vida, Santiago de Compostela, A Coruña, 15705, Spain.
Pablo PiñeiroMisión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (MBG-CSIC), Avda Vigo s/n, Campus Vida, Santiago de Compostela, A Coruña, 15705, Spain.
Rui MalhóBioISI - Biosystems and Integrative Sciences Institute, Faculty of Sciences of the University of Lisbon, Campo Grande, Lisbon, 1749-016, Portugal.
Rita Lourenço CostaInstituto Nacional de Investigação Agrária e Veterinária (INIAV), Quinta do Marquês, Avenida da República, Oeiras, 2780-159, Portugal.
Elena CorredoiraMisión Biológica de Galicia, Consejo Superior de Investigaciones Científicas (MBG-CSIC), Avda Vigo s/n, Campus Vida, Santiago de Compostela, A Coruña, 15705, Spain. elenac@mbg.csic.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Castanea sativa, a species of high ecological and economic relevance in Europe, faces severe threats from root rot caused by Phytophthora cinnamomi. To explore genetic strategies for enhancing disease tolerance, we investigated the functional role of a chestnut gene homologous to Ginkgo biloba's ginkbilobin-2 (Cast_Gnk2-like), known for its antifungal properties. Using Agrobacterium tumefaciens-mediated transformation, the Cast_Gnk2-like gene was introduced into somatic embryos from two embryogenic chestnut lines. Transformation efficiency was genotype-dependent, and varied from 14.2% to 2.5%. Twelve independent transgenic lines were confirmed by PCR, and each was estimated to carry a single copy of the transgene. Gene expression analysis revealed significant Cast_Gnk2-like transcript levels in two transgenic lines. Following cold storage and germination treatment, viable transgenic plants were regenerated. Disease tolerance assays demonstrated that Cast_Gnk2-like overexpression significantly reduced root necrosis and symptom severity, indicating enhanced tolerance to P. cinnamomi. These findings highlight the potential of targeted gene overexpression to improve disease resilience in chestnut through genetic engineering.

Indexed as

Disease ResistanceFagaceaePhytophthoraPlant DiseasesPlant ProteinsGene Expression Regulation, PlantPlant RootsPlants, Genetically ModifiedPlant ProteinsAgrobacterium tumefaciensCastanea sativaCysteine-rich repeat secretory proteinGenetic transformationInk diseaseIn vitro tolerance assaysSomatic embryos

Identifiers

PMID41514218
PMCPMC12879325

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.