Evidence map›Paper›PMID 41513003›Full record

ArticleCellular and molecular gastroenterology and hepatology2026

OGT-enriched Hepatocyte-derived Extracellular Vesicles Promote Capillarization of Liver Sinusoidal Endothelial Cells in Metabolic Dysfunction-associated Steatotic Liver Disease.

Yanjin Wang, Xulin Zhou, Yu Zhang, Fuji Yang, Yifei Chen, Yanshuang Wu, Lin Zhang, Junfei Tan, Wei Luo, Jing Zhou and 1 more

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Article in Cellular and molecular gastroenterology and hepatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yanjin WangDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China; Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Xulin ZhouDepartment of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Yu ZhangDepartment of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Fuji YangDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China; Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Yifei ChenDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China; Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Yanshuang WuDepartment of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Lin ZhangDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China; Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Junfei TanDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China; Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Wei LuoDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China.
Jing ZhouDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China.
Yongmin YanDepartment of Laboratory Medicine, Wujin Hospital Affiliated with Jiangsu University, Jiangsu University, Changzhou, China; Changzhou Key Laboratory of Exosome Foundation and Transformation Application, Wujin Hospital Affiliated with Jiangsu University (Wujin Clinical College of Xuzhou Medical University), Changzhou, China. Electronic address: yym@wjrmyy.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND &

aimsThe global incidence of metabolic dysfunction-associated steatotic liver disease (MASLD) has risen dramatically. The capillarization of liver sinusoidal endothelial cells (LSEC) represents a crucial target for intervention in MASLD. However, the regulatory mechanisms underlying LSEC capillarization in MASLD remain unclear. Angiopoietin-2 (Ang-2) serves as a key regulator of vascularization.

methodsThe role and molecular mechanism of lipotoxic hepatocyte-derived small extracellular vesicles (LTH-sEVs) on LSEC capillarization was assessed in both LSEC and high-fat diet (HFD)-induced MASLD mice. O-linked N-acetylglucosamine transferase (OGT) expression was assessed in serum sEV and liver samples from healthy individuals and patients with MASLD. The O-GlcNAcylation inhibitor Benzyl-α-GalNAc (BAGN) was utilized to HFD-induced MASLD mice.

resultsHere, we showed that LTH-sEV can upregulate the vascularization marker Ang-2 and promote LSEC capillarization in vivo and in vitro. Mechanistically, LTH-sEV may transport O-linked N-acetylglucosamine (O-GlcNAc) glycosyltransferase (OGT) to enhance the O-GlcNAc glycosylation (O-GlcNAcylation) of the hepatocyte nuclear factor 1-alpha (HNF1α) Ser471 site. This process facilitates the nuclear translocation of HNF1α and increases its transcriptional activation of Ang-2, thereby promoting the LSEC capillarization. Serum sEV derived from patients with MASLD exhibit elevated levels of OGT, which are positively correlated with alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Additionally, BAGN could dose-dependently reduce HNF1α O-GlcNAcylation, thereby alleviating LSEC capillarization and MASLD progression in HFD-induced mice.

conclusionsLTH-sEV may transport OGT to enhance HNF1α O-GlcNAcylation and activate Ang-2 expression regulated by HNF1α transcription, thus promoting LSEC capillarization. Consequently, sEV-derived OGT may serve as a novel diagnostic and therapeutic target for MASLD.

Indexed as

Endothelial CellsExtracellular VesiclesFatty LiverHepatocytesLiverN-AcetylglucosaminyltransferasesAngiopoietin-2AnimalsCapillariesDiet, High-FatDisease Models, AnimalHumansMaleMiceMice, Inbred C57BLAngiopoietin-2N-AcetylglucosaminyltransferasesOGT protein, humanOgt protein, mouseLSEC CapillarizationLTH-sEVMASLDOGT

Identifiers

PMID41513003
PMCPMC12945584

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.