ArticlePloS one2026
Comprehensive transcriptome and metabolome analysis to exploration the effects of TCs on GCs lipid metabolism at goose pre-ovulatory follicle.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Granulosa cells (GCs) and theca cells (TCs) have complex communication and interactions. Lipid metabolism in GCs is important for follicle development, which is also inseparable from the role of TCs; however, the underlying mechanisms remain unclear. In this study, we selected the pre-ovulatory (F1) follicle of geese-a poultry species with relatively low egg production-which exhibits high lipid content and vigorous lipid metabolism. Using transcriptomic and metabolomic approaches, we analyzed the effects of TCs on GCs in a co-culture model. We identified and screened the core functions and signaling pathways associated with the differentially expressed metabolites (DEMs) and genes (DEGs) between the mono-culture and co-culture groups. Key metabolites and genes within the core pathway were subsequently validated using ELISA and qPCR. Both transcriptomic and metabolomic results showed that after co-culture, the metabolite and gene expression profiles of GCs were significantly altered. Enrichment analysis revealed that both DEMs and DEGs were significantly associated with sphingolipid and glycerophospholipid metabolism pathways. In the co-culture group, metabolomic and ELISA results indicated that the concentrations of core metabolites, phosphorylcholine (PC) and phosphatidylethanolamine (PE), in GCs increased significantly (p < 0.05). Transcriptomic and qPCR results both showed that the expression levels of genes involved in PC catabolism, including phospholipase A2 epsilon-like-1, cytosolic phospholipase A2 epsilon-like-2, and phospholipase A2-like genes in GCs decreased significantly (p < 0.05). In summary, our results suggest that TCs may affect the lipid metabolism of GCs in goose F1 follicles by promoting PC synthesis within the glycerophospholipid metabolism pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.